Trends in Biotechnology
ReviewMultifunctional nanoparticles – properties and prospects for their use in human medicine
Introduction
Nanotechnology is considered by many as the next ‘big revolution’. This technological leap in controlling materials at the nanoscale has, in the past decade, driven developments enabling the use of nanodevices, such as nanoparticles, that have found applications in fields ranging from electronics and communications, through to optics, chemistry, energy and of course biology. Nanomedicine, the application of nanotechnology to healthcare, holds great promise for revolutionising medical treatments and therapies in areas, such as imaging, faster diagnosis, drug delivery and tissue regeneration, as well as the development of new medical products. Indeed, materials and devices of nanometric dimensions (1–100 nm) are already approved for clinical use and numerous products are being evaluated in clinical trials [1]. However, as discussed later, there are toxicological concerns and ethical issues that accompany nanomedicine that might cast a shadow over the promising future of this emerging field.
This article presents an overview of the current applications of nanoparticles in medicine. In particular, we focus on the development of novel multifunctional nanoparticles and illustrate their potential application in drug and gene delivery for cancer and neuropathological therapy. The current limitations of nanoparticle-based approaches are discussed, with special emphasis given to the lack of knowledge of the toxicological risks associated with the exposure to nanoparticles. Finally, we summarise the future challenges that lie ahead.
Section snippets
Multifunctional nanoparticles
Nanoparticles are constructs that possess unique physical and chemical properties associated with their being of 1–100 nm in size. The general characteristics and composition of nanoparticles are described in Box 1 and are illustrated in Figure 1. Nanometre-sized particles are in the same range of dimension as antibodies, membrane receptors, nucleic acids and proteins, among other biomolecules. These biomimetic features, together with their high surface:volume ratio and the possibility of
Current limitations to the efficacy of nanoparticles
Extensive in vivo application of nanoparticles will require a more exhaustive exploration of the physicochemical and physiological processes occurring in biological environments. For example, it is not yet possible to predict nanoparticle biodistribution according to their physicochemical properties. Moreover, nanoparticle biodistribution can be affected by undesirable interactions with biological systems and molecules, such as proteins, by a process known as opsonisation, or by the mononuclear
Concluding remarks
Although nanomedicine is a relatively new area of biotechnology, the possibilities for new therapies to treat illness and disease seem endless. Nanoparticles are already appearing in commerce as novel tools for molecular imaging, diagnosis and drug delivery formulations [4]. Of note, some nanoparticles have intrinsic therapeutic properties themselves. For example, owing to the multivalent display of ligands on their surface, dendrimers have the ability to block the binding between cells,
Acknowledgements
We acknowledge the support of the Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain.
References (82)
Emerging use of nanoparticles in diagnosis and treatment of breast cancer
Lancet Oncol.
(2006)Nanomedicine for respiratory diseases
Eur. J. Pharmacol.
(2006)Nanoparticles for bioanalysis
Curr. Opin. Chem. Biol.
(2003)Multifunctional nanocarriers
Adv. Drug Deliv. Rev.
(2006)Targeting the central nervous system: in vivo experiments with peptide-derivatized nanoparticles loaded with Loperamide and Rhodamine-123
J. Control. Release
(2007)Pegylated liposomal doxorubicin: proof of principle using preclinical animal models and pharmacokinetic studies
Semin. Oncol.
(2004)- et al.
Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles
Int. J. Pharm.
(2006) Polymers and nanoparticles: intelligent tools for intracellular targeting?
Eur. J. Pharm. Biopharm.
(2008)Nanotoxicity of iron oxide nanoparticle internalization in growing neurons
Biomaterials
(2007)Nano-C60 cytotoxicity is due to lipid peroxidation
Biomaterials
(2005)
CdSe quantum dots induce apoptosis in human neuroblastoma cells via mitochondrial-dependent pathways and inhibition of survival signals
Toxicol. Lett.
Unmodified cadmium telluride quantum dots induce reactive oxygen species formation leading to multiple organelle damage and cell death
Chem. Biol.
Nanotoxicity: the growing need for in vivo study
Curr. Opin. Biotechnol.
Investigation of the proinflammatory potential of biodegradable nanoparticle drug delivery systems in the lung
Toxicol. Appl. Pharmacol.
Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody
J. Control. Release
Thermoresponsive and biodegradable linear-dendritic nanoparticles for targeted and sustained release of a pro-apoptotic drug
Biomaterials
Magnetic-sensitive silica nanospheres for controlled drug release
Langmuir
In vitro and in vivo evaluation of a melamine dendrimer as a vehicle for drug delivery
Int. J. Pharm.
Characterization of aqueous dispersions of Fe(3)O(4) nanoparticles and their biomedical applications
Biomaterials
The pinpoint promise of nanoparticle-based drug delivery and molecular diagnosis
Biomol. Eng.
Nanoparticles in medicine: therapeutic applications and developments
Clin. Pharmacol. Ther.
The emerging nanomedicine landscape
Nat. Biotechnol.
Nanoparticle-enzyme hybrid systems for nanobiotechnology
FEBS J.
Integrated nanoparticle–biomolecule hybrid systems: synthesis, properties, and applications
Angew. Chem. Int. Ed. Engl.
Multifunctional nanorods for gene delivery
Nat. Mater.
Multifunctional nanoparticles for photothermally controlled drug delivery and magnetic resonance imaging enhancement
Small
Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced Raman spectroscopic dots
Bioconjug. Chem.
‘SMART’ drug delivery systems: double-targeted pH-responsive pharmaceutical nanocarriers
Bioconjug. Chem.
In vivo imaging of siRNA delivery and silencing in tumors
Nat. Med.
Targeted quantum dot conjugates for siRNA delivery
Bioconjug. Chem.
Fluorescent magnetic nanocrystals by sequential addition of reagents in a one-pot reaction: a simple preparation for multifunctional nanostructures
J. Am. Chem. Soc.
Multifunctional magneto-polymeric nanohybrids for targeted detection and synergistic therapeutic effects on breast cancer
Angew. Chem. Int. Ed. Engl.
Targeted nanoparticle-aptamer bioconjugates for cancer chemotherapy in vivo
Proc. Natl. Acad. Sci. U. S. A.
Organ-specific gene expression in the rhesus monkey eye following intravenous non-viral gene transfer
Mol. Vis.
Tumor-targeted gene delivery using molecularly engineered hybrid polymers functionalized with a tumor-homing peptide
Bioconjug. Chem.
Biodegradable long-circulating polymeric nanospheres
Science
In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice
Cancer Res.
Renal clearance of quantum dots
Nat. Biotechnol.
In vitro and in vivo toxicity of CdTe nanoparticles
J. Nanosci. Nanotechnol.
Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles
Nano Lett.
Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots
J. Mol. Med.
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