Elsevier

Plant Physiology and Biochemistry

Volume 87, February 2015, Pages 133-139
Plant Physiology and Biochemistry

Research article
Deterioration mechanisms in air-dry pea seeds during early aging

https://doi.org/10.1016/j.plaphy.2014.12.019Get rights and content

Highlights

  • By phosphorescence, a lot of aging pea seeds was subdivided into strong, weak and dead seeds.

  • Transformation of strong to weak seeds is not due to lipid peroxidation.

  • Transition of strong to weak seeds is related to non-enzymatic oligosaccharide hydrolysis.

Abstract

The deteriorative reactions underlying seed aging, namely, lipid peroxidation and non-enzymatic carbohydrate hydrolysis, were studied in pea seeds differing in quality. Aging air-dry seeds were subdivided to three fractions using the application to individual seeds of room temperature phosphorescence. These fractions were strong seeds (fraction I) producing normal seedlings, weak seeds (fraction II) producing mainly abnormal seedlings, and dead seeds (fraction III). Enzymatic processes cannot operate in dry seeds due to the absence of free water, and thus an analytical method was needed that does not require the addition of water. The content of lipid peroxidation products was similar in both strong and weak seeds; this excluded the possibility that lipid peroxidation induced the transition of strong to weak seeds during early aging. Lipid peroxidation was activated only in dying seeds. However, glucose content in weak seeds was much higher than in strong seeds, suggestive of non-enzymatic hydrolysis of carbohydrates, probably of oligosaccharides, which utilized bound water in air-dry seeds. This process resulted in lowered water content in weak seeds. Therefore, associated with deterioration of air-dry seeds during early aging is the non-enzymatic hydrolysis of carbohydrates, whereas lipid peroxidation is not the decisive event.

Introduction

Seed aging is usually estimated by a decrease in germination percentage measured as an average value for a seed lot. However, the aging of individual seeds occurs at various rates that results in the discrimination of aged seeds by their quality. The criteria for assessing seed quality standardized by the International Seed Testing Association (ISTA rules, 1996) are based on seed germinability. Strong seeds, i.e. seeds of high quality, produce normal seedlings, and they account for the germination percentage of a seed lot. Seed deterioration manifests itself in the appearance of weak seeds producing seedlings with some morphological abnormalities. Long-term storage of seeds results in the loss of germination ability; these seeds remain alive for some time, but do not germinate. During further aging, such seeds die.

A reliable method suitable for estimating seed quality without conducting germination tests was devised. Its advantage is that it can be applied to individual air-dry seeds and predict what will be the quality of subsequent seedling growth. The method involves the recording of room temperature phosphorescence (RTP) of individual air-dry seeds (Veselova et al., 1999, Veselova, 2002) and can discriminate between strong, weak and dead seeds. Here, strong and weak seeds are compared in order to study some key processes leading to the seed deterioration during aging.

Because these processes occur in air-dry seeds, they proceed without participation of free water, and thus are not enzymatic. Three non-enzymatic processes that are relevant to air-dry seed deterioration are lipid peroxidation, hydrolysis of carbohydrates and amino-carbonyl reactions (Murthy et al., 2003).

A common opinion is that seed aging and loss of germination capacity during artificial aging are due to accumulation of the products of free-radical lipid peroxidation (Stewart and Bewley, 1980, Smith and Berjak, 1995, McDonald, 1999, Murthy et al., 2003, Bewley et al., 2013). However, lipid peroxidation (POL) needs the participation of oxygen, which is present in mature seeds in low amounts (Borisjuk and Rolletshek, 2009). A low concentration of oxygen inside air-dry seeds is evident from a significant RTP emission because phosphorescence can be recorded only when O2 is very low (Veselova et al., 2003), oxygen being a strong quencher of phosphorescence (Frank and Pringsheim, 1943, Terenin, 1967). For this reason, active lipid peroxidation as a primary deterioration mechanism in aging dry seeds is questionable.

The next candidate offered for the role of primary aging-induced process is non-enzymatic glycosylation of proteins and amino acids (Feeney and Whitaker, 1982, Smith and Berjak, 1995, Sun and Leopold, 1995, Murthy et al., 2003). This reaction known also as the Amadori–Maillard reaction represents the interaction of the carbonyl group of glucose with the free amino group of amino acids in protein to form glycosylamine. Its subsequent rearrangement to a so-called “advanced glycosylamine end product” leads to membrane injury (Smith and Berjak, 1995, Sun and Leopold, 1995). This reaction requires the presence of reducing sugar, glucose in particular. However, the content of free glucose in air-dry pea seeds is low. Pea and soybean seeds contain about 0.1 mg glucose per g fresh weight or less (Koster and Leopold, 1988, Locher and Bucheli, 1998).

Oligosaccharides can be considered as a source of glucose, as in many aging legume seeds (Locher and Bucheli, 1998, Zalewski and Lahuta, 1998). Apparently, glucose can be formed during non-enzymatic oligosaccharide hydrolysis. This precedes glycosylation (the first step of the Amadori–Maillard reaction) because this reaction required glucose in a linear form, with a free carbonyl group (H–Cdouble bondO), and this is produced during non-enzymatic oligosaccharide hydrolysis (Stepanenko, 1977). Therefore, non-enzymatic hydrolytic oligosaccharide degradation resulting in the accumulation of glucose can be considered a likely candidate in the early deterioration of aging seeds.

The aim of this work was to study the accumulation of the products of lipid peroxidation and non-enzymatic carbohydrate hydrolysis as related to the transition of strong seeds to weak seeds at early aging.

Section snippets

Plant material

Air-dry pea seeds (Pisum sativum L., cv. Nemchinovskii-85) were received from the Institute of Grain Farming in non-chernozem soil regions (Nemchinovka, Moscow region, Russia). Seeds were stored at room temperature (20–23 °C) in open boxes for 5 years. Visually undamaged seeds of uniform size and weight of 225 ± 25 mg were selected for experiments.

Seeds stored for 0, 3, 4 and 5 years were used for aging development studies by RTP (Fig. 1), whereas seeds of 4-year long storage were analyzed by

Seed fractionation by RTP

The approach used involved RTP measurements of individual seeds, followed by an estimation of electrolyte leakage from each seed and then evaluation of its germination.

We took four air-dry seed lots, which were stored for 0, 3, 4 and 5 years. A hundred seeds were sampled from each lot, and the RTP of each seed in each lot was measured. Distribution of all these seeds by RTP is shown in (Fig. 1A). The germination percentage of these lots was expressed as the ratio of normal seedlings to total

Discussion

The advantage gained from RTP application to air-dry seeds in combination with the estimation of electrolyte leakage and seedling morphology (Fig. 1A–C) resulted in the development of a new approach, i. e. the possibility to judge in future the quality of seedlings by the analysis of dry seeds only. Therefore, all aging events occur in dry seeds but not in imbibing or germinating seeds, and it is dry seed state that must be studied to understand the causes of seed aging.

Conflicts of interest

There is no any conflict of interest.

Contribution

All authors had equal contribution to this work.

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  • Cited by (0)

    This work was supported by the Presidium of Russian Academy of Sciences [Molecular and Cell Biology Program].

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