Review ArticleBiological synthesis of metallic nanoparticles
Section snippets
Bacteria in nanoparticle synthesis
Among the milieu of natural resources, prokaryotic bacteria have been most extensively researched for synthesis of metallic nanoparticles. One of the reasons for “bacterial preference” for nanoparticles synthesis is their relative ease of manipulation. In one of the earliest studies in this technology, Slawson et al found that a silver-resistant bacterial strain isolated from silver mines, Pseudomonas stutzeri AG259, accumulated AgNPs within the periplasmic space. Of note, the particle size
Yeast in nanoparticle synthesis
Few, if any, reports exist about yeast-mediated synthesis of metallic nanoparticles. In an isolated report, Kowshik et al have demonstrated that MKY3, a silver-tolerant yeast species, when challenged with soluble silver in the log phase of growth, could precipitate a majority (>99%) of silver extracellularly as elemental nanoparticles. The size of synthesized AgNPs ranged from 2 to 5 nm. Notably, the authors used a novel procedure, based on differential thawing of the sample, for separation of
Fungi in nanoparticle synthesis
Because of their tolerance and metal bioaccumulation ability, fungi are taking the center stage of studies on biological generation of metallic nanoparticles.22 A distinct advantage of using fungi in nanoparticle synthesis is the ease in their scale-up (eg, using a thin solid substrate fermentation method). Given that fungi are extremely efficient secretors of extracellular enzymes, it is thus possible to easily obtain large-scale production of enzymes. Further advantages of using a
Plants in nanoparticle synthesis
Whereas microorganisms such as bacteria, actinomycetes, yeasts, and fungi continue to be researched and investigated in synthesis of metallic nanoparticles, the use of parts of whole plants for similar nanoparticle biosynthesis methodologies is an exciting possibility that is relatively unexplored and underexploited.26
Extracellular synthesis of AgNPs by reduction of aqueous Ag+ ions by the extract of geranium leaves (Pelargonium graveolens) has been reported.27 In comparison with the earlier
Algae in nanoparticle synthesis
Similar to yeast, there are few, if any, reports of algae being used as a “biofactory” for synthesis of metallic nanoparticles. Recently, Singaravelu et al adopted a systematic approach to study the synthesis of metallic nanoparticles by Sargassum wightii.30 This is the first report in which a marine alga has been used to synthesize highly stable extracellular gold nanoparticles in a relatively short time period compared with that of other biological procedures. Indeed, 95% of the bioreduction
Viruses in nanoparticle synthesis
Biological approaches to nanocrystal synthesis have been extended to intact biological particles. Viral scaffolds can template the nucleation and assembly of inorganic materials. Indeed, cowpea chlorotic mottle virus and cowpea mosaic virus have been used as nucleation cages for the mineralization of inorganic materials.31, 32 Furthermore, tobacco mosaic virus has been shown to direct successfully the mineralization of lead sulfide (PbS) and CdS crystalline nanowires.33 Taking the idea one step
Conclusions
A critical need in the field of nanotechnology is the development of a reliable and ecofriendly process for synthesis of metallic nanoparticles. To accomplish this, the use of natural sources like biological systems becomes essential. The synthesis of inorganic materials by biological systems is characterized by processes that occur close to ambient temperature and pressures and also at neutral pH. Of the various biological systems, bacteria are relatively easy to manipulate genetically,
Acknowledgments
We would like to thank Dr. Yogesh Shouche, Dr. Sham Diwanay, and Vasudeo Kshirsagar for their suggestions and guidance. We are grateful to Dr. Leonard D. Kohn (Ohio University, Athens, Ohio) and Dr. Nilesh M. Dagia (Piramal Life Sciences Limited, Mumbai, India) for editorial assistance with the manuscript. We also wish to thank colleagues at the National Center for Cell Science (Pune, India) and Abasaheb Garware College (Pune, India) for their constant support and encouragement.
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