Albumin nanoparticles as nanocarriers for drug delivery: Focusing on antibody and nanobody delivery and albumin-based drugs
Graphical abstract
Introduction
Albumin is a protein mostly known for its therapeutic application in drug delivery and disease diagnosis [1]. Through its various features and advantages, it has caught researcher attentions in the field of medical nanotechnology [2]. Due to its accessibility, it is highly used as a pattern in chemical and physical studies [2,3]. Albumin is extracted from different sources such as Ovalbumin (OVA), Bovine Serum Albumin (BSA), Human Serum Albumin (HSA) and Rat Serum Albumin (RSA) [3,4]. According to recent studies albumin can bind to a broad range of molecules and substances including therapeutic drugs, antibodies and peptides [[5], [6], [7]]. This special feature introduced albumin as an important carrier in drug deliveries [[5], [6], [7]](Fig. 1). Albumin has various physiological roles in humans and improves the overall functions of every individual [8]. Specific and efficient drug delivery is a fundamental challenge in clinical therapies and is shifting attentions to carriers such as albumin in recent years [8,9]. The aim of this study is to review features of various albumins and their applications as nanocarriers focusing on antibody and nanobody delivery. Furthermore, different approaches in synthesizing albumin nanoparticles will be discussed. Meanwhile, we will examine albumin as a nanocarrier and review different studies regarding albumin carriers. There are different physicochemical approaches for producing albumin nanoparticles that are used based on the target of study [10,11]. Some of these approaches will be discussed in the following.
Section snippets
OVA
OVA is the most common types of food proteins frequently used in the food industries. It is a glycoprotein with 47 kDa molecular weight and 365 amino acid and one disulfide bond [12]. The main reason in choosing OVA as a drug carrier is advantages like easy access to its sources and its low price [3,11]. Features like pH and temperature sensitivity makes OVA a potential drug carrier [[13], [14], [15]]. OVA can increase MHC class I and induce lymphocytes activation. OVA can be used as a carrier
Physiologic roles of albumin
Albumin is one of the most important proteins in plasma with various vital roles. It consists 40% of the protein mass of plasma and has an amount of 35–50 g in every liter of serum [9,36]. Albumin is responsible for the 80% of osmotic pressure alone [4,37]. In addition, it has a role in pH maintenance through working as a buffer [4]. Albumin is known as a carrier of numerous molecules like fatty acids, eicosanoids, biliary acid, steroid hormones, vitamin D and C, fulate, copper, zinc, calcium,
Methods for producing albumin nanoparticles
There are different physical and chemical methods for producing albumin nanoparticles. Common chemical methods are desolvation, emolation and self-assembly [8,10,40]. Thermal gelation, nanoparticle albumin-bound (NAB) technology and nanospray drying are physical methods in producing albumin nanoparticles [11,41] (Table 1). Furthermore, there are different approaches for loading albumin nanoparticles on targeted drugs (for instance, covalent bonds, surface coating and electrostatic absorption).
Albumin as a nanocarrier
Albumin has unique features that make it a suitable option as a drug transporter. Some of them are as follow:
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A great amount of albumin is already in our body, therefore injecting too much albumin would have lower side effects than other carriers [1].
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Transporting therapeutic drugs with albumin not only reduces costs but also decreases drug's toxicity [63].
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The bond between albumin and hydrophobic substances are reversible which facilitates transporting drug in the body and releasing it onto the
Utilizing albumin to improve functionality of the available drugs in the market
There are numerous albumin-based drug delivery systems. Albumin is utilized in various disease treatments such as diabetes, cancer and rheumatoid arthritis.
Alburx®: This drug contains 5–25% human albumin and is administered intravenously. Alburx® serves as a substitute for albumin upon heavy blood loss due to traumas like severe burn or laceration. In addition, it can be used during operations, dialysis, liver or pancreatic failure. This product is produced from the plasma of the American blood
Conclusion
Through recent years, albumin nanoparticles were the most important and promising nanoparticles in treating many diseases such as cancer. According to the aim of study, different types of albumin with different characteristics can be used. In the past years, albumin nanoparticles were used as nanocarriers. Studies show that conjugating albumin to drugs increases their stability and efficiency while reducing their toxicity. Different studies that conjugated albumin carriers in the in vivo and in
Author contribution
EK prepared draft of manuscript. MB red and edited the manuscript. FKL conceptualized and improved the manuscript.
Declaration of competing interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
This work was supported by Pasteur Institute of Iran, Tehran, Iran.
References (116)
Albumin's role in steroid hormone action and the origins of vertebrates: is albumin an essential protein?
FEBS Lett.
(1998)Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles
Biomaterials
(2014)- et al.
Impact of albumin on drug delivery—new applications on the horizon
J. Control. Release
(2012) - et al.
Efficient delivery of therapeutic agents by using targeted albumin nanoparticles
- et al.
Albumin-based nanoparticles as potential controlled release drug delivery systems
J. Control. Release
(2012) - et al.
Structure and properties of ovalbumin
J. Chromatogr. B Biomed. Sci. Appl.
(2001) Structural properties of condensed ovalbumin systems following application of high pressure
Food Hydrocolloids
(2016)Phase separation behavior and structural analysis of ovalbumin–gum Arabic complex coacervation
Food Hydrocolloids
(2015)Unfolding and refolding of bovine serum albumin induced by cetylpyridinium bromide
Biophys. J.
(2005)- et al.
Engineered and designed peptide-based fibrous biomaterials
Curr. Opin. Solid State Mater. Sci.
(2004)
Bovine serum albumin: an efficient biomacromolecule nanocarrier for improving the therapeutic efficacy of chrysin
J. Mol. Liq.
Probing the cysteine 34 residue in human serum albumin using fluorescence techniques
Biochim. Biophys. Acta Protein Struct. Mol. Enzymol.
Pseudo-esterase activity of human albumin slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines
J. Biol. Chem.
Albumin synthesis
Characterization and side effect analysis of a newly designed nanoemulsion targeting human serum albumin for drug delivery
Colloids Surfaces B Biointerfaces
Poly (ethylene glycol)–human serum albumin–paclitaxel conjugates: preparation, characterization and pharmacokinetics
J. Control. Release
Alteration of human serum albumin binding properties induced by modifications: a review
Spectrochim. Acta A Mol. Biomol. Spectrosc.
Serum albumin
Desolvation process and surface characterisation of protein nanoparticles
Int. J. Pharm.
Long-circulating self-assembled cholesteryl albumin nanoparticles enhance tumor accumulation of hydrophobic anticancer drug
Eur. J. Pharm. Biopharm.
Albumin nanoparticles for the delivery of gabapentin: preparation, characterization and pharmacodynamic studies
Int. J. Pharm.
HI 6 human serum albumin nanoparticles—development and transport over an in vitro blood–brain barrier model
Toxicol. Lett.
Optimization of the preparation process for human serum albumin (HSA) nanoparticles
Int. J. Pharm.
Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties
Eur. J. Pharm. Sci.
Nano spray drying: a novel method for preparing protein nanoparticles for protein therapy
Int. J. Pharm.
Nanoparticle albumin bound (nab) technology: targeting tumors through the endothelial gp60 receptor and SPARC
Nanomed. Nanotechnol. Biol. Med.
Nanoparticle albumin-bound (nab™)-paclitaxel: improving efficacy and tolerability by targeted drug delivery in metastatic breast cancer
Eur. J. Canc. Suppl.
DNA-polycation nanospheres as non-viral gene delivery vehicles
J. Control. Release
Prodrug encapsulated albumin nanoparticles as an alternative approach to manifest anti-proliferative effects of suicide gene therapy
Mater. Sci. Eng. C
Nanoparticles in cancer therapy and diagnosis
Adv. Drug Deliv. Rev.
Nanotechnological approaches for the delivery of macromolecules
J. Control. Release
Paclitaxel and di-fluorinated curcumin loaded in albumin nanoparticles for targeted synergistic combination therapy of ovarian and cervical cancers
Colloids Surfaces B Biointerfaces
A novel self-assembly albumin nanocarrier for reducing doxorubicin-mediated cardiotoxicity
J. Pharm. Sci.
A novel lipoprotein-mimic nanocarrier composed of the modified protein and lipid for tumor cell targeting delivery
J. Control. Release
Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy
J. Control. Release
In vivo and in vitro biocompatibility study of novel microemulsion hybridized with bovine serum albumin as nanocarrier for drug delivery
Heliyon
Strategies for extended serum half-life of protein therapeutics
Curr. Opin. Biotechnol.
An overview on application of phage display technique in immunological studies
Asian Pacific J. Trop. Biomed.
Inhibition of angiogenesis in human endothelial cell using VEGF specific nanobody
Mol. Immunol.
Generation of llama single-domain antibodies against methotrexate, a prototypical hapten
Mol. Immunol.
Nanobodies as novel therapeutic agents in envenomation
Biochim. Biophys. Acta Gen. Subj.
Nanobody-albumin nanoparticles (NANAPs) for the delivery of a multikinase inhibitor 17864 to EGFR overexpressing tumor cells
J. Control. Release
Targeting hepatocyte growth factor receptor (Met) positive tumor cells using internalizing nanobody-decorated albumin nanoparticles
Biomaterials
Albumin as a drug delivery and diagnostic tool and its market approved products
Acta Pol. Pharm.
Reviews: the role of albumin in human physiology and pathophysiology, Part III: albumin and disease states
J. Parenter. Enteral Nutr.
All about Albumin: Biochemistry, Genetics, and Medical Applications
Albumin as a delivery carrier for rheumatoid arthritis
J Nanomed Nanotechol
The novel albumin–chitosan core–shell nanoparticles for gene delivery: preparation, optimization and cell uptake investigation
J. Nanoparticle Res.
Albumin nanostructures as advanced drug delivery systems
Expert Opin. Drug Deliv.
Antigen delivery with poly (propylacrylic acid) conjugation enhances MHC-1 presentation and T-cell activation
Bioconjug. Chem.
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