Quencher-free molecular beacon: Enhancement of the signal-to-background ratio with graphene oxide

doi:10.1016/j.bmcl.2010.12.004

Bioorganic & Medicinal Chemistry Letters

Volume 21, Issue 2, 15 January 2011, Pages 704-706

https://doi.org/10.1016/j.bmcl.2010.12.004 Get rights and content

Abstract

We report the highly improved version of quencher-free molecular beacon (QF-MB) system by using graphene oxide (GO) as an external quencher. This QF-MB/GO system provided a higher S/B ratio (31.0) relative to that (2.2) of the same system in the absence of GO, while retaining a high selectivity for fully matched over single-base-mismatched targets.

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Acknowledgments

We thank MEST/NRF for financial support through the CRI-Acceleration Research (Laboratory for Fluorescent Nucleic Acid Systems), Gene Therapy R&D, EPB Center programs, NRF [GRL, WCU(R32-2008-000-10180-0), EPB(R11-2008-052-01000), BK21], and KISTI (KSC-2008-K08-0002). Professor B. H. Hong is acknowledged for advice about GO.

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Bioorganic & Medicinal Chemistry Letters

Abstract

Graphical abstract

Section snippets

Acknowledgments

References and notes (7)

Science

Nature

Nat. Nanotechnol.

Angew. Chem., Int. Ed.

Adv. Funct. Mater.

Chem. Commun.

Chem. Eur. J.

Chem. Commun.

Nat. Biotechnol.

Science

Angew. Chem. Int. Ed.

Chem. Soc. Rev.

Curr. Opin. Chem. Biol.

Cited by (30)

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Density functional theory calculations and molecular dynamics simulations of the adsorption of ellipticine anticancer drug on graphene oxide surface in aqueous medium as well as under controlled pH conditions

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Quencher-free molecular aptamer beacons (QF-MABs) for detection of ATP

Graphene materials-based energy acceptor systems and sensors