Elsevier

Aquaculture Reports

Volume 17, July 2020, 100408
Aquaculture Reports

Beneficial impacts on growth, intestinal health, immune responses and ammonia resistance of pacific white shrimp (Litopenaeus vannamei) fed dietary synbiotic (mannan oligosaccharide and Bacillus licheniformis)

https://doi.org/10.1016/j.aqrep.2020.100408Get rights and content
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Highlights

  • The growth and feed utilization of the shrimp could be enhanced by the dietary MOS and Bacillus licheniformis or their combination.

  • Supplemented diets could positively altered intestinal morphology and short-chain fatty acids (SCFAs).

  • Supplemented diets could enhance shrimp’s immune responses during the feeding period.

  • There were no interaction effects on growth, intestinal health, immune responses between MOS and Bacillus licheniformis.

Abstract

The impacts of dietary mannan oligosaccharide (MOS) and Bacillus lincheniformis (B. lincheniformis) on growth performance, immune responses, intestinal health and ammonia resistance of Litopenaeus vannamei were explored. Four resultant diets (control diet (CON), 0.2 % MOS (MOS), 0.1 % B. lincheniformis (BL), 0.2 % MOS plus 0.1 % BL (SYN)) were produced to feed shrimps for 8 weeks. Results showed that significantly higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and protein productive value (PPV) were found in shrimp fed the SYN diet (P < 0.05). The villus number (VN) and villus height (VH) of shrimps fed the SYN diet were significantly higher than those of shrimps fed the CON diet (P <  0.05). Moreover, significantly thicker submucosa (SM) of shrimp fed the MOS diet than that of shrimps fed BL and CON diets were found (P <  0.05). Intestinal acetic acid content was significantly higher in shrimps fed supplemented diets (P <  0.05). Meanwhile, the propionic acid content in shrimps fed BL and SYN diets were significantly higher than those in shrimps fed the other diets (P <  0.05). The expression levels of catalase (CAT), glutothion peroxidase (GPX), superoxide dismutase (SOD), penaeidin -3a (Pen-3a) and heat shock protein (Hsp-70) were significantly upregulated by supplemented diets at some time points (P <  0.05). The survival rates of shrimps fed supplemented diets after ammonia challenge were significantly higher (P <  0.05). The expression levels of CAT, SOD, Pen-3a and Hsp-70 in shrimps fed supplemented diets were upregulated after ammonia challenge. In conclusion, dietary synbiotic could enhance growth performance, feed utilization and intestinal morphology, while dietary MOS and/or B. lincheniformis supplementation could positively influence the intestinal SCFAs content, increase immune responses and ammonia resistance of Litopenaeus vannamei.

Keywords

MOS
Bacillus lincheniformis
Intestinal morphology
SCFAs
Litopenaeus vannamei

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