Involvement of an O2 generating system in the induction of necrotic lesions on tobacco leaves infected with tobacco mosaic virus

https://doi.org/10.1016/S0885-5765(88)80013-4Get rights and content

Leaf discs of the tobacco cultivar Samsun NN [a cultivar of Nicotiana tabacum containing the N gene for resistance to tobacco mosaic virus (TMV)], inoculated with TMV and grown at 30 °C for 36 h to allow infection, formed ring-shaped necrotic lesions 6–7 h after transfer to 20–26 °C. The infected leaf discs showed a marked O2 generating activity almost immediately after transfer to 20 °C, which decreased rapidly and then underwent a cyclic oscillation three times within a further 4 h incubation at 20 °C. The net activity detected for the initial 10 min after transfer to 20 °C was significantly correlated with the number of necrotic lesions which appeared later. Leaves of non-infected Samsun NN, and those of TMV-infected and non-infected Samsun (a cultivar without the N gene) showed little O2 generating activity after transfer from 30 to 20 °C.

The accumulation of formazan at the sites of subsequent lesion development in TMV-infected Samsun leaf discs, infiltrated with nitroblue tetrazolium (NBT) at the beginning of the 20 °C incubation, suggested that O2 generation may be activated in sites of TMV-multiplication.

A membrane-rich fraction isolated from TMV-infected Samsun NN leaf discs incubated at 30 °C showed an enhanced O2 generating activity dependent on NADPH and Ca2+ at 20–26 °C, but not at temperatures higher than 28 °C. Infiltration of TMV-infected Samsun NN leaves with NADP+, SOD or catalase caused a reduction in necrotic lesion formation following transfer from 30 to 20 °C.

These results indicate that the activation of an NADPH-dependent O2 generating reaction in leaves of TMV-infected tobacco cultivars carrying the N gene may be involved in the induction of the necrotic lesions caused by virus infection.

References (24)

  • ChaiH.B. et al.

    Superoxide anion generation: A response of potato leaves to infection with Phytophthora infestans

    Phytopathology

    (1987)
  • ElstnerE.F.

    Oxygen activation and oxygen toxicity

    Annual Review of Plant Physiology

    (1982)
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