Baculovirus multigene expression vectors and their use for understanding the assembly process of architecturally complex virus particles☆
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Viruses and virus-like particles in biotechnology: Fundamentals and applications
2019, Comprehensive BiotechnologyEvaluation of the immune response afforded by a subunit vaccine candidate against bluetongue virus in mice and sheep
2018, Veterinary MicrobiologyCitation Excerpt :The downside to bacterial expression systems is the lack of post-translational modification pathways such as the glycosylation, phosphorylation and acylation found in eukaryotes (Georgiou and Valax, 1996). Previous investigations indicate that the baculovirus system is capable of producing sufficient protein for vaccine generation (Mikhailov et al., 1996; Roy et al., 1997; Verma et al., 1998). In the current research, the baculovirus system was selected as a starting point for a series of experiments to evaluate the immune response produced by the proposed vaccine candidate.
Synthesis of empty african horse sickness virus particles
2016, Virus ResearchViruses and Virus-Like Particles in Biotechnology: Fundamentals and Applications
2011, Comprehensive Biotechnology, Second EditionProduction of protein complexes via co-expression
2011, Protein Expression and PurificationCitation Excerpt :Using deletion constructs for each of the heterotrimeric components of the yeast Piccolo NuA4 histone acetyltransferase (HAT) complex, Song and colleagues [95] dissected regions of the component proteins responsible for nucleosome-specific HAT activities via a combinatorial co-expression approach. Deletion and/or mutation constructs have also been used to identify proper constructs for crystallization [85], or to define critical regions in component proteins responsible for assembly of complex bluetongue VLPs [96]. For proteins predicted to have intrinsically disordered regions (intrinsically unstructured or regions with reduced complexity), inclusion of a specific region might be needed to facilitate complex formation, since these regions are rich with post-translational modification sites and are often involved in interactions with other proteins/domains [97].
New baculovirus expression tools for recombinant protein complex production
2010, Journal of Structural Biology
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Presented at the International Conference on ‘Eukaryotic Expression Vector Systems: Biology and Applications’, National Institute of Immunology, New Delhi, India, 4–8 February 1996.