LetterQuantitative assessment of human erythrocyte membrane solubilization by Triton X-100
Section snippets
Acknowledgements
Grants from Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) and Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Proc. 01/03632-0) are gratefully acknowledged.
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2022, Bioresource TechnologyCitation Excerpt :During this process, the product can be extracted by the extractive phase according to the product-surfactant affinity, shown in Fig. 2b (Banik et al., 2003; Wu et al., 2017; Chen et al., 2018a; Huang et al., 2019). This process is also called “membrane solubilization” and has been reported when used zwitterionic surfactants (Preté et al.; 2002b), non-ionic surfactants as Triton X-100 (Preté et al., 2002a), and alkyl ethers (Domingues et al., 2008) in model membrane cells (Manaargadoo-Catin et al., 2016). Membrane solubilization can be also used as an extractive method after bioprocess (Wu et al., 2017).
Dosing metric in cellular experiments: The mol/cell metric has its limitations
2022, Toxicology in VitroPolymeric micelles of pluronic F127 reduce hemolytic potential of amphiphilic drugs
2019, Colloids and Surfaces B: BiointerfacesCitation Excerpt :The effect of a drug on the erythrocyte membrane can be attributed to two main phenomena: (i) its insertion into the membrane and (ii) the intensity of the membrane-perturbing action of the molecule [6]. The hemolytic process induced by amphiphilic compounds can be described as a bilayer-to-micelle transition depending on the amphiphile:lipid ratio, in which the concentration of amphiphilic compound required to induce membrane saturation (the onset of hemolysis) and total membrane solubilization (100% lysis) determines the limits for the co-existence of mixed-membranes and mixed-micelles [7,8]. Important events resulting from the interaction of amphiphilic drugs with cell membranes include: (i) change in bilayer organization affecting permeability, change in membrane proteins structure and function; (ii) lipid or drug flip-flop; (iii) change in cells shape (iv) endo/exovesiculation; (v) non-bilayer drug-lipid domains (non-bilayer phase formation); (vi) interdigitation; and (vii) membrane disruption and even solubilization [5].
Molecular features of nonionic detergents involved in the binding kinetics and solubilization efficiency, as studied in model (Langmuir films) and biological (Erythrocytes) membranes
2018, Colloids and Surfaces B: BiointerfacesCitation Excerpt :It was proposed by Griffin, late in the 1940s [33], being extensively used to describe detergent series. Preté et al. [8] showed that within the homologous CnEm series, the greater the length of the hydrocarbon chain and the lower the HLB, the greater the lytic potential of the detergent. Similar results were shown with homologous series of Renex [6], ASB [5] and other detergents [6,34,35].