Elsevier

Theriogenology

Volume 53, Issue 5, 15 March 2000, Pages 1093-1103
Theriogenology

Development of infantile rat ovaries autotransplanted after cryopreservation by vitrification

https://doi.org/10.1016/S0093-691X(00)00255-7Get rights and content

Abstract

We cryopreserved infantile rat ovaries by vitrification and assessed their viability by autotransplantation. Hemilateral ovarian transplantation was performed on rats on postnatal Days 10 to 12. The left ovary of each rat was dissected out, cryopreserved by vitrification using a modified vitrification solution (VS1), and then autotransplanted under the capsule of the right kidney. The right ovary of each rat was removed. For the control, the left ovary was dissected out from each rat and was immediately transplanted by the same procedure, without cryopreservation. Rats were nursed until weaning, and then the day of vaginal opening, estrous cyclicity from the day of vaginal opening until postnatal Day 84, and histology of ovarian grafts at postnatal Day 84 were examined. The time course of development of endocrine function of cryopreserved grafts was similar to that of fresh grafts. In ovarian transplants recovered on postnatal Day 84, antral follicles and corpora lutea (CL) were observed in addition to small follicles, although the number of antral follicles in cryopreserved grafts was smaller than in the fresh grafts. These results indicate that cryopreservation of ovarian tissue by vitrification can be used for the preservation of fertility and endocrine function of ovaries.

References (41)

  • CoxS-L et al.

    Transplantation of cryopreserved fetal ovarian tissue to adult recipients in mice

    J Reprod Fertil

    (1996)
  • DeaneslyR

    Immature rat ovaries grafted after freezing and thawing

    J Endocrinol

    (1954)
  • DeaneslyR

    Cyclic function in ovarian grafts

    J Endocrinol

    (1956)
  • DeaneslyR

    Egg survival in immature rat ovaries grafted after freezing and thawing.

  • Del VecchioFR

    Normal development, growth, and aging of the female genital tract

  • DinnyesA et al.

    Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification or slow freezing methods

    Mol Reprod Dev

    (1995)
  • GosdenRG et al.

    Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196 °C

    Hum Reprod

    (1994)
  • GreenSH et al.

    The numbers of oocytes in ovarian autografts after freezing and thawing

    J Endocrinol

    (1956)
  • GunasenaKT et al.

    Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice

    Biol Reprod

    (1997)
  • GunasenaKT et al.

    Critser JK Live birth after autologous transplant of cryopreserved mouse ovaries

    Hum Reprod

    (1997)
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