Elsevier

Methods in Enzymology

Volume 303, 1999, Pages 179-205
Methods in Enzymology

[12] DNA arrays for analysis of gene expression

https://doi.org/10.1016/S0076-6879(99)03014-1Get rights and content

Publisher Summary

This chapter describes one of the currently used microarray technologies commonly called “spotting” or “printing” because DNAs are physically spotted on a solid substrate in which short oligonucleotides is synthesized directly on a solid support. In standard spotting applications, large collections of DNA samples are assembled in 96- or 384-well plates. DNA microarrays are used for a variety of purposes; essentially any property of a DNA sequence that can be made experimentally to result in differential recovery of that sequence can be assayed for thousands of sequences at once by DNA microarray hybridization. The chapter focuses on the application of DNA microarrays to gene expression studies and discusses general principles of whole genome expression monitoring as well as detailing the specific process of making and using spotted DNA microarrays.

References (24)

  • F. Forozan et al.

    Trends Genet.

    (1997)
  • R.J. Sapolsky et al.

    Genomics

    (1996)
  • E.M. Southern

    J. Mol. Biol.

    (1975)
  • E.M. Southern et al.

    Genomics

    (1992)
  • G. Lennon et al.

    Genomics

    (1996)
  • J. DeRisi et al.

    Nature Genet.

    (1996)
  • M. Chee et al.

    Science

    (1996)
  • J.L. DeRisi et al.

    Science

    (1997)
  • R.A. Heller et al.
  • D.A. Lashkari et al.
  • L. Wodicka et al.

    Nature Biotechnol.

    (1997)
  • M. Schena et al.
  • Cited by (0)

    View full text