Dextran-coated silica packings for high-performance size-exclusion chromatography of proteins
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Ionic liquid-modified mesoporous silica stationary phase for separation of polysaccharides with size exclusion chromatography
2018, Separation and Purification TechnologyCitation Excerpt :Silica was used as the stationary phase in size exclusion chromatography in the 1970s. Porous silica and dextran-coated silica have been used as the stationary phase in size exclusion chromatography for the analysis and purification of proteins and biopolymers [11,12]. Yu et al. filled a column with spherical particles with a pore size of 250 and 500 Å to determine the separation capacity of size exclusion chromatography.
Polar silica-based stationary phases. Part III- Neutral silica stationary phase with surface bound maltose for affinity chromatography at reduced non-specific interactions
2017, Journal of Chromatography ACitation Excerpt :In addition, these composite materials protect the silica matrix against chemical attacks by aggressive mobile phases, and consequently increasing the lifespan of the column [15]. So far, some attempts have been made for the coating of silica supports with different carbohydrates including agarose [16], dextran [17–20], cellulose [21], chitosan [22,23], glucose [24] and maltose [25] (see also the preceding paper in this issue). In most of these reported works, carbohydrate layers were coated on the silica surface by using non-covalent approaches leading to leaching out of the sugar layer with the attached affinity ligands, and in turn compromising the reusability of the HPAC stationary phases.
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