New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

doi:10.1016/S0021-9673(00)93969-4

Journal of Chromatography A

Volume 411, 1987, Pages 177-184

https://doi.org/10.1016/S0021-9673(00)93969-4 Get rights and content

Abstract

A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography. The new resin is a quadridentate chelate former and specially suitable for metal ions with coordination numbers of six, since two valencies remain for the reversible binding of biopolymers. It has been found to chelate Cu²⁺ and Ni²⁺ strongly and to be superior to the known iminodiacetic acid adsorbent. Charged with Ni²⁺ it was evaluated for the ability to bind peptides and proteins containing neighbouring histidine residues. The remarkable specificity found makes it an attractive addition to the range of adsorbents for metal chelate affinity chromatography.

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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Abstract

J. Chromatogr.

Trends Biotechnol.

J. Biol. Chem.