Elsevier

Journal of Chromatography A

Volume 215, 23 October 1981, Pages 131-141
Journal of Chromatography A

Separation of membrane components by partition in detergent-containing polymer phase systems : Isolation of the light harvesting chlorophyll a/b protein

https://doi.org/10.1016/S0021-9673(00)81394-1Get rights and content

Abstract

The application of phase partition, using Triton X-100 in combination with the aqueous two-phase system dextran-polyethylene glycol, is described for the separation and isolation of the chlorophyll-protein complexes which are the main hydrophobic proteins of the chloroplast thylakoid membrane. The chlorophyll a protein of photosystem I is easily extracted into the upper phase while the chlorophyll a/b proteins of the light-harvesting complex are more resistant to extraction, thus remaining at the interface. The separation method can easily be scaled up into a preparative method. Essentially pure light-harvesting complex from spinach chloroplast could be obtained within 3 h.

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