Separation of membrane components by partition in detergent-containing polymer phase systems : Isolation of the light harvesting chlorophyll a/b protein
References (25)
- et al.
Biochim. Biophys. Acta
(1975) Endeavour
(1977)J. Chromatogr.
(1978)Appl. Biochem. Bioeng.
(1979)- et al.
Biochem. Biophys. Res. Commun.
(1974) - et al.
J. Biol. Chem.
(1975) Biochim. Biophys. Acta
(1976)- et al.
FEBS Lett.
(1976) - et al.
J. Biol. Chem.
(1978) - et al.
J. Steroid Biochem.
(1973)
J. Immunol. Methods
Methods Enzymol.
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Use of aqueous two-phase PEG-salt systems for the removal of anionic surfactant from effluents
2017, Journal of Environmental ManagementCitation Excerpt :It was observed that the partitioning behavior of the surfactants was strongly influenced by their charge. Albertsson and Andersson (Albertsson and Andersson (1981) and Svensson et al. (1985) described the partitioning of Triton X-100 and/or octyl glucoside surfactants incorporated into a PEG-dextran ATPS, for the purpose of separating and isolating chlorophyll proteins of the chloroplast thylakoid membranes. A review of the literature reveals that there is a lack of experimental data concerning the interactions responsible for the partitioning of surfactants in ATPS.
Pigments protect the light harvesting proteins of chloroplast thylakoid membranes against digestion by gastrointestinal proteases
2011, Food HydrocolloidsCitation Excerpt :Thylakoid membrane proteins have been extensively characterized by SDS–PAGE and the molecular weight of the monomers of the different intrinsic membrane protein complexes is well known (Barros & Kuhlbrandt, 2009; Liu et al., 2009; Nelson & Ben-Sham, 2004; Schmid, 2008) and also the location of the monomers in the SDS–PAGE gels (Andreasson et al., 1988; Emek et al., 2010). Isolated LHC, the major pigment complex, shows two bands around 25–27 kD in the gel (Andersson & Albertsson, 1981). In addition the capacity of the treated thylakoids to inhibit lipase/colipase activity was determined.
Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo<inf>3</inf> ubiquinol oxidase, in a detergent-polymer aqueous two-phase system containing metal-chelating polymer
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2000, Biochimica et Biophysica Acta - General SubjectsProtein partitioning in weakly charged polymer-surfactant aqueous two-phase systems
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