Elsevier

Experimental Neurology

Volume 83, Issue 2, February 1984, Pages 443-447
Experimental Neurology

Research note
Parent and daughter axon density in the limb stump and regenerating limb bud of the newt

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Abstract

Axonal density in the amputated limb stump and regenerating limb bud of the newt was determined by axon counts and planimetry of silver-stained sections at various distances from the amputation level. Traumatic degeneration occurred for at least 250 μm proximal to the amputation level. Axonal density at 250 μm distal to the amputation was less than half that at 100 μm. Attempts to increase in situ axonal density in limbs of animals naturally incapable of limb regeneration can be based on specific knowledge of natural traumatic degeneration and sprouting prior to axonal manipulation.

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1

The authors acknowledge the excellent histological preparations of the material by Kari Maier. We are indebted to Richard Grimm and K. Maier for assistance with axonal counting. This study was supported by National Institutes of Health NS 15731-02.

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