Superoxide dismutase, catalase and cell dimorphism in Candida albicans cells exposed to methanol and different temperatures

https://doi.org/10.1016/1367-8280(94)90089-2Get rights and content

Abstract

The combined effects of methanol and different temperatures on Candida albicans were studied. Growth curve, cell morphology, superoxide dismutase (SOD) and catalase activity levels have been determined. Cell growth in each medium was comparable to 28°C annd 37°C. The growth rate was not affected by methanol, in the presence of glucose, while it was much lower in the absence of sugar. Cell dimorphism appeared after thermic stress and it was also dependent on the medium composition. In all media, both SOD and catalase levels were much higher at 37°C. The presence of methanol per se did not affect the enzymatic levels, while the absence of glucose gave higher SOD levels.

References (23)

  • A. Battistoni et al.

    Temperature-dependent protein folding in vivo—lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli

    Biochem. biophys. Res. Commun.

    (1992)
  • Cited by (13)

    • A role for antioxidants in acclimation of marine derived pathogenic fungus (NIOCC 1) to salt stress

      2012, Microbial Pathogenesis
      Citation Excerpt :

      Catalase (EC 1.11.1.6) catalyzes the degradation of hydrogen peroxide with release of molecular oxygen. It has been found that over expression of SOD and CAT provides protection from lethal heat shock in Saccharomyces cerevisiae and C. albicans [71,72]. In addition, a protective role of antioxidant enzymes (SOD, CAT, and peroxidase) against temperature treatment of fungal cells (N. crassa and A. nidulans) has also been demonstrated [73,74] suggesting that the production of toxic oxygen derivatives increased in biotic and abiotic stress conditions.

    • Oxidative stress response in Paracoccidioides brasiliensis: assessing catalase and cytochrome c peroxidase

      2008, Mycological Research
      Citation Excerpt :

      Our results showed that catalase activity is higher in cells grown to the stationary phase than in cells from the exponential phase. The reported value for catalase specific activity in Candida albicans grown to the exponential phase is 250 U mg−1 protein (Romandini et al. 1994) and in exponential Saccharomyces cerevisiae cells is 0.65 U mg−1 protein (César-Ferreira 2002). Catalase activity in P. brasiliensis seems comparable with that found in some vertebrate tissues, such as liver, kidney, and adipose tissue (200–1500 U mg−1 protein) (Hermes-Lima 2004).

    View all citing articles on Scopus
    View full text