Use of a fibre optic immunosensor for the detection of pesticides

doi:10.1016/0925-4005(92)80354-Z

Sensors and Actuators B: Chemical

Volume 7, Issues 1–3, March 1992, Pages 509-512

https://doi.org/10.1016/0925-4005(92)80354-Z Get rights and content

Abstract

The principle of a competitive immunoassay was employed for the development of an optical immunosensor for pesticide analysis. Triazines were assayed using fluorescence labelled antibodies. On one end of a hard clad silica fibre, which was stripped of cladding, a derivative of the s-triazines, aminohexylatrazine, was immobilized. The fibre was inserted in a flow through cuvette. Triazine antibodies conjugated with fluoresceine isothiocyanate (FITC) were detected after binding to the fibre surface by the fluorescence coupled into the fibre via the evanescent field. In the presence of triazines the fluorescence signal decreases due to the inhibition of antibody binding to the fibre. The detection limit of the sensor for terbutryn was 0.1 ng/ml (obtained using a monoclonal antibody). This is the maximum concentration of a single pesticide which may be present in drinking water, according to European Community regulations.

References (10)

J.M. Van Emon et al.
Immunoassay techniques for pesticide analysis
Anal. Methods Pestic. Plant Growth Regul.
(1989)
P. Krämer et al.
Flow injection immunoanalysis (FIIA) — a new immunoassay format for the determination of pesticides in water
Biosensors Bioelectron.
(1991)
H.H. Weetall
Covalent coupling methods for inorganic support materials
B. Hock
Immunoassay Study Group, Enzyme immunoassays for the determination of s-triazines in water samples: two interlaboratory tests
Anal. Lett.
(1991)
W. Stöcklein et al.
Flow injection immunoanalysis (FIIA) for the determination of pesticides in water
GBF Monographs
(1989)

There are more references available in the full text version of this article.

Cited by (59)

Polypyrrole-neutravidin layer for impedimetric biosensor
2008, Sensors and Actuators, B: Chemical
Polypyrrole synthesis and neutravidin entrapment inside the polymer were performed simultaneously in one step using a controlled anodic current passing. The incorporation of neutravidin within the electrogenerated film was confirmed by FTIR and impedance spectroscopy. Biotinylated anti-triazine Fab fragment was attached to the PPy/neutravidin modified electrode throughout the well-studied biotin–neutravidin interaction. During impedance measurements, the charge transfer resistance increased gradually with atrazine concentration whereas no significant response was recorded after rabbit IgG injection. The immunosensor was very sensitive to atrazine antigen in the range of 0.1–200 ng/ml and the detection limit attained 0.1 ng/ml.
Optrode-based fiber optic biosensors (bio-optrode)
2008, Optical Biosensors
Optrode-based fiber optic biosensors (bio-optrodes) are analytical devices incorporating optical fibers and biological recognition molecules. Optical fibers are small and flexible ‘wires’ made out of glass or plastic that can transmit light signals, with minimal loss, over long distances. The light signals are generated by a sensing layer, which is usually composed of biorecognition molecules and dyes, coupled to the fiber end. Light is transmitted through the optical fibers to the sensing layer where different optical phenomena such as absorption or luminescence are used to measure the interactions between the analyte and the sensing layer. Bio-optrodes can be used for remote analytical applications including clinical, environmental, and industrial process monitoring. This chapter describes the basic characteristics of optical fibers and the optical methods used to transduce a biorecognition event to an optical signal. It also summarizes the instrumentation employed in optrode biosensors, the biological sensing elements, and the methods to immobilize them on the fiber optic surfaces. Furthermore, it illustrates few examples of new bio-optrode technologies and applications, which include nanotechnological, clinical, environmental and industrial applications. Recent advancements in bio-optrode technologies include the development of nanoscale bio-optrodes, enabling measurements inside single living cells, and the development of multianalyte and reagentless bio-optrodes. The study concludes by discussing the advantages and limitations of bio-optrodes.
Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
2003, Biosensors and Bioelectronics
We present a new optical biosensor for probing molecular binding to a water/glass interface. The system is designed to measure the kinetics of surface reactions down to low analyte concentrations straightforwardly. The selective detection of surface bound fluorescence is achieved by collecting supercritical angle fluorescence (SAF) emission of surface bound molecules into the glass. Thereby the expansion of the detection volume into the aqueous probe is reduced to about one sixth of the fluorescence wavelength, consequently bulk fluorescence from the solution is rejected successfully. The SAF-signal is captured by a parabolic glass lens, which leads to high spatial collection efficiency and detection sensitivity. The sensor has an inverted optical design and is compatible with common glass cover slips, which strongly facilitates operation for the user working in the biological and biochemical fields. The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10⁻¹³ mol/l.
Visible optical sensing of ammonia based on polyaniline film
2003, Sensors and Actuators, B: Chemical
In this work, a new optical gas sensor using thin polymer layers was developed. The polymer sensing layers were synthesized by chemical polymerization using the distilled aniline. The optical property of a polymer as a sensing material was analyzed using UV-Vis-NIR. These layers were prepared using centrifugal force. The polymer layer was uniformly deposited on inner glass pillar. The light source was red-light emitting diode (LED) with a 655 nm wavelength. The light power transmitted through the guide cell was measured with a photodiode. Variation of the light power transmitted was detected when the gas was absorbed into the polymer layer. The transmitted light intensity decreased because gas molecules were absorbed into the polymer layer. This optical sensor showed a good sensitivity to NH₃ gas.
Surface plasmon resonance based on Bragg gratings to test the durability of Au-Al films
2002, Materials Letters
Life extension for metallic aircraft are becoming increasingly important as budgets shrink and world economies realign themselves to an uncertain future. One approach to reducing this budget is to develop a reliable method to detect and monitor corrosion. This paper presents analysis and testing of fiber optic corrosion sensor based on Bragg reflection influenced by surface plasmon resonance (SPR) measurement. Fiber Bragg gratings have been inscribed into the core. The sensing element of the fiber sensor based on Bragg gratings has been fabricated by depositing a thin layer of highly reflecting metal onto the fiber core, 15-nm thick, of aluminum over 30-nm layer of gold. The coatings have been produced by thermal evaporation and by phase vapor deposition, respectively. A technique that uses the surface plasmon resonance phenomenon offers a sensitive method for studying the durability of these films. Tests include exposing gold, and gold–aluminum and gold–thiol films to air, and hydrogen sulfide and water.
Immunosensors for pesticide determination in natural waters
2001, TrAC - Trends in Analytical Chemistry
The development and application of immunosensors for environmental monitoring has grown steadily in recent years. In this review, immunosensors developed in the last few years are highlighted regarding their importance and practical contribution to environmental analysis with particular emphasis on monitoring of pesticide levels. Different transduction elements and mechanisms used for the detection of the physicochemical change(s) produced by the biological interaction are shown, as well as their application to the development of real-time measurement devices or systems. In addition, examples concerning the analysis of pesticides in natural water samples obtained using a recently developed optical immunosensor are given. Validation of immunosensor measurements with conventional chromatographic techniques is also reported. The immunosensor system detection mechanism is based on a solid-phase fluoroimmunoassay combined with an optical transducer chip chemically modified with an analyte derivative. Applications to the analysis of atrazine, simazine, paraquat, alachlor, 2,4-D and isoproturon in water are also reviewed.

View all citing articles on Scopus

View full text

Use of a fibre optic immunosensor for the detection of pesticides

Abstract

Anal. Methods Pestic. Plant Growth Regul.

Biosensors Bioelectron.

Immunoassay Study Group, Enzyme immunoassays for the determination of s-triazines in water samples: two interlaboratory tests

Anal. Lett.

Flow injection immunoanalysis (FIIA) for the determination of pesticides in water

GBF Monographs