Cloning of a structural and functional homolog of the circadian clock gene period from the giant silkmoth antheraea pernyi

doi:10.1016/0896-6273(94)90054-X

Neuron

Volume 13, Issue 5, November 1994, Pages 1167-1176

https://doi.org/10.1016/0896-6273(94)90054-X Get rights and content

Abstract

The period (per) gene of Drosophila plays an important role in circadian clock function. Interestingly, homologs of per have not been cloned outside of dipteran species. Using a PCR strategy, we now report the cloning of the cDNA of a per homolog from the silkmoth Antheraea pernyi. The cDNA encodes a protein of 849 amino acids, which shows highest identity (39%) with the per protein of Drosophila virilis. Stretches of high identity between moth and fly proteins are in the amino terminus, the PAS region, and the region surrounding the site of the per^s mutation in Drosophila. Moth per homolog mRNA levels exhibit a prominent circadian variation in adult heads, and per protein antibodies show a pronounced variation of per antigen staining in photoreceptor nuclei. With sequence information derived from moth and flies, per-like cDNA fragments were readily cloned by PCR from other moth species and a third insect order.

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Neuron

ArticleCloning of a structural and functional homolog of the circadian clock gene period from the giant silkmoth antheraea pernyi

Abstract

Neuron

Anal. Biochem.

Neuron

Neuron

Neuron

Dev. Biol.

Genetic, molecular, and cellular studies of the per locus and its products in Drosophila melanogaster

Cloning of the Ah-receptor cDNA reveals a distinctive ligand-activated transcription factor

A family of unusually spliced biologically active transcripts encoded by a Drosophila clock gene

Nature

Interspecific comparison of the period gene of Drosophila reveals large blocks of non-conserved coding DNA

EMBO J.

The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product

Cell

Temporal phosphorylation of the Drosophila period gene

Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms

J. Neurosci.

Transplantation of a circadian pacemaker in Drosophila

Nature

Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels

Nature

Cloning of a factor required for activity of the Ah (Dioxin) receptor

Science

PAS is a dimerization domain common to Drosophila period and several transcription factors

Nature

Article
Cloning of a structural and functional homolog of the circadian clock gene period from the giant silkmoth antheraea pernyi