Neuron
Volume 3, Issue 6, December 1989, Pages 677-688
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Article
Motor nerve terminal loss from degenerating muscle fibers

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Abstract

The fate of nerve terminals following elimination of postsynaptic target cells was studied in living mouse muscle. Several days after muscle fiber damage, observations of previously identified neuromuscular junctions showed that motor nerve terminal branches had rapidly disappeared from degenerating muscle fibers. Following muscle fiber regeneration, loss of terminal branches ceased and nerve terminals regrew, reestablishing some of the original sites and adding new branches. The distribution of acetylcholine receptors reorganized in the regenerated muscle so that perfect alignment was reestablished with the newly configured nerve terminals. These results argue that the maintenance of the full complement of nerve terminal branches at a neuromuscular junction is dependent on the presence of a healthy muscle fiber. Similarly, regenerating muscle is dependent on the nerve terminal for the organization and maintenance of postsynaptic receptors.

References (33)

  • E. Frank et al.

    Persistence of junctional acetylcholine receptors following degeneration

  • M.A. Glicksman et al.

    Differentiation of motor nerve terminals formed in the absence of muscle fibres

    J. Neurocytol.

    (1983)
  • I. Jirmanova

    Ultrastructure of motor end-plates during pharmacologically-induced degeneration and subsequent regeneration of skeletal muscle

    J. Neurocytol.

    (1975)
  • K.H. Jones et al.

    An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide

    J. Histochem. Cytochem.

    (1985)
  • M.J. Karnovsky et al.

    A “direct coloring” thiocholine method for cholinesterase

    J. Histochem. Cytochem.

    (1964)
  • T.L. Lentz

    Development of the neuromuscular junction. II. Cytological and cytochemical studies on the neuromuscular junction of dedifferentiating muscle in the regenerating limb of the newt triturus

    J. Cell Biol.

    (1970)
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