Original contributionA comparative study of EPR spin trapping and cytochrome c reduction techniques for the measurement of superoxide anions
References (48)
- et al.
Oxygen radicals, inflammation, and tissue injury
Free Radic. Biol. Med.
(1988) - et al.
An overview of the relationship between oxidative stress and chemical carcinogenesis
Free Radic. Biol. Med.
(1991) Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
Free Radic. Biol. Med.
(1990)- et al.
Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions
Anal. Biochem.
(1987) Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase
J. Biol. Chem.
(1970)- et al.
The reduction of cytochrome c by milk xanthine oxidase
J. Biol. Chem.
(1968) - et al.
The kinetics of the reduction of cytochrome c by the superoxide anion radical
Biochim. Biophys. Acta
(1976) - et al.
The reaction between the superoxide anion radical and cytochrome c
Biochim. Biophys. Acta
(1975) - et al.
Free radical reduction of ferricytochrome-c
Biochem. Biophys. Res. Commun.
(1975) - et al.
How relevant is the reoxidation of ferrocytochrome c by hydrogen peroxide when determining superoxide anion production?
FEBS Lett.
(1988)
The utility of superoxide dismutase in studying free radical reactions, II. The mechanism of the mediation of cytochrome c reduction by a variety of electron carriers
J. Biol. Chem.
One-electron transfer reactions in biochemical systems VIII. Kinetic study of superoxide dismutase
Biochim. Biophys. Acta
The extinction coefficient of cytochrome c
Biochim. Biophys. Acta
Phosphate, not superoxide dismutase, facilitates electron transfer from ferrous salts to cytochrome c
Arch. Biochem. Biophys.
Reduction and destruction rates of nitroxide spin probes
Arch. Biochem. Biophys.
Spin trapping of superoxide and hydroxyl radical: Practical aspects
Arch. Biochem. Biophys.
A critical review of spin trapping in biological systems
Use of spin traps in biological systems
Adv. Free Radic. Biol. Med.
Spin trapping: ESR parameters of spin adducts
Free Radic. Biol. Med.
Oxidation of hydroxylamines to nitroxide spin labels in living cells
Biochim. Biophys. Acta
Electron paramagnetic resonance evidence that cellular oxygen toxicity is caused by the generation of superoxide and hydroxyl free radicals
FEBS Lett.
The cellular-induced decay of DMPO spin adducts of ·OH and ·O2−
Free Radic. Biol. Med.
Insight into the nature and site of oxygen-centered free radical generation by endothelial cell monolayers using a novel spin trapping technique
Blood
Do human neutrophils make hydroxyl radical?
J. Biol. Chem.
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2012, Free Radical Biology and MedicineCitation Excerpt :This technique utilizes the reaction of nitrone spin traps with O2•− resulting in the production of relatively stable spin adducts [15]. Our previous studies showed that the EPR spin trapping technique is more sensitive than the cytochrome c reduction method in quantifying O2•− [44,48]. However, the application of the EPR spin trapping method for very low O2•− fluxes is still limited because of slow spin trap reactivity with O2•−, and short adduct half-life, as well as decreased EPR analytical sensitivity due to the complex multiline EPR spectra of the spin adduct.
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