Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes

doi:10.1016/0891-5849(88)90023-8

Free Radical Biology and Medicine

Volume 4, Issue 3, 1988, Pages 155-161

https://doi.org/10.1016/0891-5849(88)90023-8 Get rights and content

Abstract

Liver slices were used to measure, lipid peroxidation induced by bromotrichloromethane, tert-butyl hydroperoxide (t-BOOH), or ferrous iron. The responses of liver homogenates and microsomes to oxidative conditions were compared with the response of tissue slices. Lipid peroxidation was evaluated by the production of thiobarbituric acid-reactive substances (TBARS). As was observed in homogenates and microsomes, TBARS production by liver slices depended upon the amount of tissue, the incubation time, inducer, the amount of inducer, and the presence of antioxidant. Control liver slices incubated at 37°C for 2 h produced 19 nmol of TBARS per g of liver. When slices were incubated in the presence of 1 mM BrCC1₃, 1 mM t-BOOH, or 50 μM ferrous iron, TBARS production increased 4.6-, 8.2-, or 6.7-fold over the control value, respectively. Comparable induction of TBARS by liver homogenates and microsomes was observed when these preparations were incubated with the same inducers. Addition of 5 μM butylated hydroxytoluene (BHT)_prevented the induction of TBARS by 50 μM ferrous iron byliver slices. The results indicate the usefulness of tissue slices to measure lipid peroxidation. The usefulness of tissue slices is emphasized when a number of compounds or tissues are studied and tissue integrity is desired as in toxicological, pharmacological, and nutritional studies where reduced numbers of experimental animals is a relevant issue.

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Free Radical Biology and Medicine

Abstract

References (24)

Release of ethane and pentane from rat tissue slices: effect of vitamin E, halogenated hydrocarbons, and iron overload

Arch. Biochem. Biophys.

Halogenated hydrocarbons and hydroperoxide-induced lipid peroxidation in rat tissue slices

J. Free Rad. Biol. Med.

Halogenated compounds as inducers of lipid peroxidation in tissue slices

J. Free Rad. Biol. Med.

Hyperoxia increases oxygen radical production in rat lungs and lung mitochondira

J. Biol. Chem.

Production of volatile hydrocarbons by isolated hepatocytes: an in vitro model for lipid peroxidation studies

Toxicol. Appl. Pharmacol.

The measurement of lipid peroxidation in isolated hepatocytes

Biochem. Pharmacol.

Cellular toxicity and lipid peroxidation in response to mercury

Toxicol. Appl. Pharmacol.

A simple fluorometric assay for lipoperoxide in blood plasma

Biochem. Med.

Role of metals in oxygen radical reactions

J. Free Rad. Biol. Med.

Methods of studying secretion in vivo and in liver slices

Lipid peroxidation in rat brain cortical slices as measured by the thiobarbituric acids test

J. Neurochem.

Partial inactivation of Na-K-ATPase in cortical brain slices incubated in normal Krebs-Ringer phosphate medium at 1 and 10 Atm oxygen pressures

J. Neurochem.

Cited by (639)

Prolonged hypothermic machine perfusion enables daytime liver transplantation – an IDEAL stage 2 prospective clinical trial

Anthropogenic underwater noise: A review on physiological and molecular responses of marine biota

Hake heads proteins hydrolysates as a source of bioactive peptides with prevention against hypercholesterolemia in mice: The involvement of oxidative dysfunction in hepatic tissues

Protective effects of β-caryophyllene on mitochondrial damage and cardiac hypertrophy pathways in isoproterenol-induced myocardial infarcted rats

Heat stress, especially when coupled with high light, accelerates the decline of tropical seagrass (Enhalus acoroides) meadows

Senna petersiana (Bolle) leaf extract modulates glycemic homeostasis and improves dysregulated enzyme activities in fructose-fed streptozotocin-induced diabetic rats

Original contributionLipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes

Abstract

Arch. Biochem. Biophys.

J. Free Rad. Biol. Med.

J. Free Rad. Biol. Med.

J. Biol. Chem.

Toxicol. Appl. Pharmacol.

Biochem. Pharmacol.

Toxicol. Appl. Pharmacol.

Biochem. Med.

J. Free Rad. Biol. Med.

Methods of studying secretion in vivo and in liver slices

Lipid peroxidation in rat brain cortical slices as measured by the thiobarbituric acids test

J. Neurochem.

Partial inactivation of Na-K-ATPase in cortical brain slices incubated in normal Krebs-Ringer phosphate medium at 1 and 10 Atm oxygen pressures

J. Neurochem.

Original contribution
Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes