Elsevier

Toxicology Letters

Volume 24, Issues 2–3, February–March 1985, Pages 119-124
Toxicology Letters

Toxicity determined in vitro by morphological alterations and neutral red absorption

https://doi.org/10.1016/0378-4274(85)90046-3Get rights and content

Abstract

A method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible assays lend themselves to a screening procedure of potential toxicants which can help reduce the use of animals for toxicity testing.

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