Elsevier

Gene

Volume 97, Issue 2, 15 January 1991, Pages 289-293
Gene

Short communication
Isolation and sequence of a cDNA encoding mouse IMP dehydrogenase

https://doi.org/10.1016/0378-1119(91)90065-JGet rights and content

Abstract

Inosinic acid (IMP) dehydrogenase (IMPD) catalyzes the conversion of IMP to XMP as the first committed step in GMP biosynthesis de novo. We have isolated a cDNA containing the complete coding region of mouse IMPD by its ability to complement a bacterial mutant lacking IMPD activity. Two independent cDNA clones were isolated by complementation. of which the longest was 1.7 kb in length. Northern analyses, using the IMPD cDNA as a probe, indicated that mature IMPD mRNA was a single species approx 2.0 kb in size. Mouse IMPD is almost identical to Chinese hamster and human IMPDs and is highly conserved between Escherichia coli and mouse, with a direct amino acid (aa) identity of 39%, which increases to 60%, if conserved aa are considered. The leader region of our longest cDNA clone is G + C-rich and contains two tandem copies of a G + C-rich direct repeal.

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    Present address: Department of Molecular Genetics, University of Texas, MD Andersen Hospital and Tumor Institute, 1515 Holcombe Blvd., Houston, TX 77030 (U.S.A.) Tel. (713 792-8940.

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