Elsevier

Gene

Volume 43, Issue 3, 1986, Pages 305-310
Gene

Short communication
A rapid and simple procedure for the preparation of homogeneously labeled DNA probes

https://doi.org/10.1016/0378-1119(86)90221-0Get rights and content

Abstract

This report describes a novel technique for the preparation of homogeneously labeled DNA probes. The procedure uses a heteroduplex intermediate, which is directly prepared from a double-stranded plasmid, and therefore requires neither sub-cloning of the fragment of interest into a specialized vector nor the use of a synthetic primer. The technical advantages of the procedure are demonstrated by preparation of a probe able to determine the precise location and efficiency of utilization of the polyadenylation site used during transcription of the rat preproinsulin II gene in a transfected avian cell line.

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