Short communicationPowerful and versatile enhancer-promoter unit for mammalian expression vectors
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Cited by (249)
Olfactory receptor coding sequences cause silencing of episomal constructs in multiple cell lines
2021, Molecular and Cellular NeuroscienceCitation Excerpt :Perhaps consistent with this second model, it has been observed that precocious OR expression driven by a heterologous promoter results in that OR being selected more frequently within the nasal epithelium (Nguyen et al., 2007), suggestive of an iterative process whereby a “head start” might provide a particular OR gene a selective advantage. In this study, we produced constructs with OR coding sequences driven by a heterologous cytomegalovirus (CMV) promoter (Boshart et al., 1985; Foecking and Hofstetter, 1986; Nelson et al., 1987), and upstream of an IRES-GFP (Martı́nez-Salas, 1999) for visualization of expressing cells within transfected populations. We transfected two types of immature OSN cell types (OP6, OP27; Illing et al., 2002), as well as a GD25 fibroblast-derived cell line (Fässler et al., 1995).
Neuronal Activation Stimulates Cytomegalovirus Promoter-Driven Transgene Expression
2019, Molecular Therapy Methods and Clinical DevelopmentCitation Excerpt :Due to strong expression and compatibility with numerous cell types, promoters derived from the enhancer region of the cytomegalovirus (CMV) immediate early (IE) promoter are among the most common for general expression vectors.1,2
Improving transcriptional activity of human cytomegalovirus major immediate-early promoter by mutating NF-κB binding sites
2018, Protein Expression and PurificationCitation Excerpt :When the human and mouse MIEPs were used to produce the monoclonal antibody (mAb) in the Chinese hamster ovary (CHO) cell, the human MIEP had the expression level higher than the mouse MIEP [8]. Originally, the human MIEP has been identified to display high transcriptional activity in a wide range of cultured cells [9]. The human MIEP can drive the ectopic gene expression in broad tissues in transgenic animals [10,11].
Effect of adenovirus infection on transgene expression under the adenoviral MLP/TPL and the CMVie promoter/enhancer in CHO cells
2017, Journal of Genetic Engineering and BiotechnologyEfficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry
2015, Journal of Bioscience and Bioengineering