Elsevier

Biochimie

Volume 70, Issue 12, December 1988, Pages 1709-1713
Biochimie

Research article
Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis

https://doi.org/10.1016/0300-9084(88)90028-4Get rights and content

Abstract

The purpose of this study was to confirm the photoprotective effect on skin of vitamins A and E, due to inhibition of polyamine synthesis and production of free radicals. These variables were measured in the lumbar epidermis of the female hairless mouse subjected to UVA + B irradiation. Polyamines were assayed in epidermal homogenate by HPLC, and production of oxygenated free radicals was determined by spectrofluorimetric assay of malonyl dialdehyde. It was determined that butyl-hydroxy-toluene and vitamin E inhibited production of free radicals (56% and 60%, respectively) and caused a significant reduction in polyamine biosynthesis (P<0.01), whereas the inhibitory effect of and caused a significant reduction in polyamine biosynthesis (P<0.01), whereas the inhibitory effect of malonyl dualdehyde induced by vitamin A (30%) had no associated effect on polyamine metabolism.

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