Determination of monosaturated fatty acid double-bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyl disulphide adducts

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Abstract

Monounsaturated fatty acid double-bond position and geometry have been determined for microbial monocultures and complex microbial consortia by capillary GC-MS of their dimethyl disulphide (DMDS) adducts. The technique has permitted (i) chromatographic separation and positive identification of adducts derived fromcis/trans isomers, (ii) characterization of long-chain monounsaturated components (up to 26:1), and (iii) the identification of a wide range of monounsaturated components derived from methanotrophic soil material. The methanotrophic soil sample contained a high relative proportion of the novel phospholipid ester-linked fatty acid 18:1Δ 10c. The DMDS procedure offers a simple and rapid approach that can be routinely applied to microbial fatty acids derived from environmental samples and monocultures.

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Present address: CSIRO Marine Laboratories, Division of Oceanography, GPO Box 1538, Hobart, Tasmania 7001, Australia.

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