Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Interaction of polycations with cell-surface negative charges of epithelial cells
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2018, European Polymer JournalCitation Excerpt :Besides, calcium phosphate ceramics have limitations in terms of having inherent brittleness and difficulty in processing [11]. Among synthetic biodegradable polymers, cationic polymers have come into prominence due to their enhanced interactions with the negatively charged cell membrane [12] under physiological pH, allowing cell penetration [13] and cell surface adhesion capabilities [14]. On the other hand, the high density of positively charged functional groups may make such polymers cytotoxic.
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2017, Sensors and Actuators, B: ChemicalCitation Excerpt :PLL has an excellent cell capture capability and strong irreversible adherence to metal-oxide surfaces. Various studies have documented that several cell lines, among others, fibroblast and epithelial cells possess an overall negative surface charge, and this facilitates their adhesion to positively charged surface [53,54]. Similarly, B cell membranes also have negative charges, which even increase with maturation [55], therefore the use of positively charged poly-l-lysine was a sensible selection.
Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
2015, Biosensors and BioelectronicsCitation Excerpt :Various studies have documented that adherent cell lines such as fibroblast and endothelial cells possess an overall negative surface charge, which facilitates their adhesion to positively charged surface (Somosy et al., 1986; Singh et al., 1992; Hamdan et al., 2006). This negative surface charge may be attributed to the carboxylic groups of aspartic and glutamic acid (pKa=3.9–4.2) of proteins, carboxylic groups of sialic acid (pKa=2.8) of glycoproteins, phosphate groups (pKa=2.0), and sulfate groups (pKa=1.9) of sulphated proteoglycans or glycoproteins (Singh et al., 1992). Another explanation is that their extracellular matrix is rich in negatively charged glucosaminoglycans (Somosy et al., 1986), thus suggesting that the observed cell adhesion behavior is mainly electrostatic in nature.
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2012, Carbohydrate PolymersCitation Excerpt :This kind of elastic fluids in liquid crystal state made them potentially favorable for cells growth and proliferation. In the PU/LC composite, rich-OPC domains would be more elastic in comparison to rich-PPC regions due to the longer flexible branches of OPC molecules, and consequently facilitated cell adhesion (Ryser, 1967; Singh, Kasinath, & Lewis, 1992; Woltman et al., 2007). When LC was at low concentration, the LC formed discrete small size island-like droplets dispersing throughout the composite membrane surfaces.
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