The comet assay: a comprehensive review

doi:10.1016/0165-1110(94)00013-3

Mutation Research/Reviews in Genetic Toxicology

Volume 339, Issue 1, February 1995, Pages 37-59

https://doi.org/10.1016/0165-1110(94)00013-3 Get rights and content

Abstract

The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored.

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The comet assay: a comprehensive review

Abstract

Mutation Res.

FEBS lett.

Mutation Res.

J Photochem. Photobiol. B. Biology

FEMS Microbiol. Lett.

Cancer Lett.

Cancer Lett.

Cell

Anal. Biochem.

Pharmacol. Ther.

Mutation Res.

Mutation Res.

Mutation Res.

J Chromatogr.

Mutation Res.

Mutation Res.

Mutation Res.

Mutation Res.

Mutation Res.

Mutation Res.

Mutation Res.

Biochem. Biophys. Res. Commun.

Mutation Res.

Calibration of pulsed-field gel electrophoresis for measurement of DNA double-strand breaks

Int. J Radiat. Biol.

Techniques to measure single-strand breaks in cells: A review

Int. J Radiat. Biol.

Radiation-induced single-strand breaks in DNA determined by rate of alkaline strand separation and hydroxyapatite chromatography: an alternative to velocity sedimentation

Int. J. Radiat. Biol.

Single cell gel electrophoresis on leukocytes of patients with precancerous and cancerous lesions of the cervix

Am. J Hum. Gen.

Fas transduces activation signals in normal human T-lymphocytes

J Exp. Med.

Dietary carcinogens and anticarcinogens

Science

Apoptosis: the role of the endonuclease

Am. J Pathol.

Hypersensitivity of human lymphocytes to UV-B and solar irradiation

Cancer Res.

The role of oxygenation in embryotoxic mechanisms of three bioreducible agents

Teratology

Comparative studies on cytotoxic and genotoxic effects of two organic mercury compounds in lymphocytes and gastric mucosa cells of Sprague-Dawley rats

Env. Mol. Mutagen.

Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects

Mutation Res.

Effect of linker length on DNA-binding affinity, cross-linking efficiency and cytotoxicity of C8-linked pyrrolobenzodiazepine dimers

J Chem. Soc. Chem. Commun.

X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution

Nucleic Acids Res.

SR4233 (Tirapazamine): a new anticancer drug exploiting hypoxia in solid tumours

Br. J. Cancer

Prooxidant states and tumor promotion

Science

An introduction to free radical biochemistry

Br. Med. Bull.

Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA

Carcinogenesis

Genotoxicity testing: recent advances and future trends

Chem. Indust.

Supercoils in human DNA

J Cell Science

Human gastric carcinogenesis: a multi-step and multi-factorial process — First American Cancer Society award lecture on cancer epidemiology and prevention

Cancer Res.

Heterogeneity of radiation-induced damage measured by microgel electrophoresis

Int. J Radiat. Biol.

DNA damage in rat islet cells and HIT-T15 cells exposed to interleukin-1β and nitric oxide- studies using the comet assay

Diabetologia

Induction of apoptosis — new targets for cancer chemotherapy

Sem. Cancer Biol.

Macrophage recognition of cells undergoing programmed cell death (apoptosis)

Immunology

DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines

Radiat. Res.