A fluorometric assay of peroxidase activity utilizing 2′,7′-dichlorofluorescein with thiocyanate: application to the study of salivary secretion
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Effects of molecular weight of hyaluronic acid on its viscosity and enzymatic activities of lysozyme and peroxidase
2018, Archives of Oral BiologyCitation Excerpt :The experiments for measuring enzymatic activities of HEWL and salivary lysozyme were performed eight times in duplicate. Peroxidase activity was determined by the method described previously (Hannig et al., 2008, Hannig, Spies, Spitzmüller, & Hannig, 2010; Hannig, Spitzmüller et al., 2010; Proctor & Chan, 1994). In the presence of peroxidase and hydrogen peroxidase, fluorogenic 2′,7′-dichlorofluorescein (LDCF) is oxidized to fluorescent dichlorofluorescein (DCF).
Enzyme activities in parotid saliva of patients with the restrictive type of anorexia nervosa
2017, Archives of Oral BiologyCitation Excerpt :Extinction was read at γ = 405 nm at 25 °C. Peroxidase activity was determined as described in detail previously (Hannig et al., 2008; Proctor & Chan, 1994). In the presence of peroxidase and hydrogen peroxide, fluorogenic 2,7-dichlorofluorescein (LDCF) is converted to fluorescent dichlorofluorescein (DCF).
Salivary secretion of immunoglobulin A by submandibular glands in response to autonomimetic infusions in anaesthetised rats
2003, Journal of NeuroimmunologyThe intracellular oxidation of 2′,7′-dichlorofluorescin in murine T lymphocytes
2001, Free Radical Biology and MedicineProtein secretion from rat submandibular acini and granular ducts: Effects of exogenous VIP and substance P during parasympathetic nerve stimulation
1998, Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology