MyoD or Myf-5 is required for the formation of skeletal muscle

doi:10.1016/0092-8674(93)90621-V

Cell

Volume 75, Issue 7, 31 December 1993, Pages 1351-1359

https://doi.org/10.1016/0092-8674(93)90621-V Get rights and content

Abstract

Mice carrying null mutations in the myogenic regulatory factors Myf-5 or MyoD have apparently normal skeletal muscle. To address whether these two factors functionally substitute for one another in myogenesis, mice carrying mutant Myf-5 and MyoD genes were interbred. While mice lacking both MyoD and Myf-5 were born alive, they were immobile and died soon after birth. Northern blot and S1 nuclease analyses indicated that Myf-5(-/-);MyoD(-/-) mice expressed no detectable skeletal muscle-specific mRNAs. Histological examination of these mice revealed a complete absence of skeletal muscle. Immunohistochemical analysis indicated an absence of desmin-expressing myoblast-like cells. These observations suggest that either Myf-5 or MyoD is required for the determination of skeletal myoblasts, their propagation, or both during embryonic development and indicate that these factors play, at least in part, functionally redundant roles in myogenesis.

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Abstract

References (31)

The protein ld: a negative regulator of helix-loop-helix DNA binding proteins

Cell

Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death

Cell

Expression of RAPsyn (43K protein) and nicotinic acetylcholine receptor genes is not coordinately regulated in mouse muscle

Neuron

In vitro and in vivo expression of α7 integrin and desmin define primary and secondary myogenic lineages

Dev. Biol.

cDNA clone and expression analysis of rodent fast and slow troponin I mRNAs

J. Biol. Chem.

FGF inactivates myogenic helix-loop-helix proteins through phosphorylation of a conserved protein kinase C site in their DNA-binding domains

Cell

Myoblast diversity in skeletal myogenesis: how much and to what end?

Cell

Inactivation of MyoD in mice leads to up-regulation of the myogenic HLH gene Myf-5 and results in apparently normal muscle development

Cell

Mast cells are closely apposed to nerves in the human gastrointestinal mucosa

Gastroenterology

Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD

Cell

The family of mouse phosphoglycerate kinase gene and pseudogenes

Somat. Cell Mol. Genet.

Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA

Eur. J. Biochem.

Theory and Practice of Histological Techniques

The muscle regulatory gene Myf-6 has a biphasic pattern of expression during early mouse development

J. Cell Biol.

Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products

EMBO J.

Cited by (1405)

Parallel control of cold-triggered adipocyte thermogenesis by UCP1 and CKB

Dietary selenium promoted muscle growth (myofiber hypertrophy rather than hyperplasia) by reducing endoplasmic reticulum stress in juvenile grass carp (Ctenopharyngodon idella)

N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway

Effects of proanthocyanidins and dialdehyde chitosan on the proliferation and differentiation of bovine myoblast for cultured meat production

Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus)

Acetyl genistin modulates myotube differentiation and attenuates dexamethasone-induced muscle atrophy through the FoxO1/3 signaling pathway in C2C12 myotubes

Cell

ArticleMyoD or Myf-5 is required for the formation of skeletal muscle

Abstract

Cell

Cell

Neuron

Dev. Biol.

J. Biol. Chem.

Cell

Cell

Cell

Gastroenterology

Cell

The family of mouse phosphoglycerate kinase gene and pseudogenes

Somat. Cell Mol. Genet.

Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA

Eur. J. Biochem.

Theory and Practice of Histological Techniques

The muscle regulatory gene Myf-6 has a biphasic pattern of expression during early mouse development

J. Cell Biol.

Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products

EMBO J.

Article
MyoD or Myf-5 is required for the formation of skeletal muscle