Cell
Volume 60, Issue 4, 23 February 1990, Pages 557-563
Journal home page for Cell

PTC is a novel rearranged form of the ret proto-oncogene and is frequently detected in vivo in human thyroid papillary carcinomas

https://doi.org/10.1016/0092-8674(90)90659-3Get rights and content

Abstract

We recently detected a novel activated oncogene by transfection analysis on NIH 3T3 cells in five out of 20 primary human thyroid papillary carcinomas and in the available lymph node metastases. We designated this transforming gene FTC (for papillary thyroid carcinoma). Here we describe the molecular cloning and sequencing of the gene. The new oncogene resulted from the rearrangement of an unknown amino,terminal sequence to the tyrosine kinase domain of the ret proto-oncogene. This gene rearrangement was detected in all of the transfectants and In all of the original tumor DNAs, but not in normal DNA of the same patients, thus indicating that this genetic lesion occurred in vivo and is specific to somatic tumors. Moreover, the transcript coded for by the fused gene was detected in an additional FTC-positive human papillary carcinoma for which mRNA was available.

References (31)

  • M. Grieco et al.

    Molecular cloning of PTC, a new oncogene found activated in human thyroid papillary carcinomas and their lymph-node metastases

    Ann. NY Acad. Sci.

    (1988)
  • P. Heitz et al.

    Thyroid cancer

    Cancer

    (1976)
  • T.V. Huynh et al.

    Constructing and screening cDNA libraries in lambda gt10 and lambda gt11

  • F. Ishikawa et al.

    Rat c-raf oncogene activation by a rearrangement that produces a fused protein

    Mol. Cell. Biol.

    (1987)
  • Y. Ishizaka et al.

    Activation of the ret-II oncogene without a sequence encoding a transmembrane domain and transforming activity of two rot II oncogene products differing in carboxy4ermini due to alternative splicing

    Oncogene

    (1989)
  • Cited by (0)

    View full text