The E. coli dnaY gene encodes an arginine transfer RNA

doi:10.1016/0092-8674(86)90331-4

Cell

Volume 45, Issue 3, 9 May 1986, Pages 453-459

https://doi.org/10.1016/0092-8674(86)90331-4 Get rights and content

Abstract

The E. coli dnaY gene product is an arginine tRNA. Its 77 nucleotide sequence can be folded into a typical cloverleaf structure with a UCU anticodon corresponding to the rare arginine codon AGA. A dnaY⁺ plasmid confers overproduction of at least one arginine-accepting minor species of tRNA^Arg. The dnaY promoter was identified by run-off transcription studies, and the initiating nucleotide was identified by sequencing the 5′ end of the in vitro transcript. The primary products in vitro are RNAs of 180 and 190 nucleotides, which presumably are processed in vivo to generate the mature form. Transcription is terminated in vitro, and presumably in vivo, by a rhodependent process.

References (50)

C. Andrews et al.
Transcription termination factor rho mediates simultaneous release of RNA transcripts and DNA template from complexes with Escherichia coli RNA polymerase
J. Biol. Chem.
(1985)
M.S. Boguski et al.
Identification of a cytidine-specific ribonuclease from chicken liver
J. Biol. Chem.
(1980)
K. Bovre et al.
Techniques of RNA-DNA hybridization in solution for the study of viral transcription
Meth. Virol.
(1971)
T.F.R. Celis et al.
Studies on the mechanism of arginine biosynthesis in Escherichia coli. IV. Further studies on the role of arginine transfer RNA repression of the enzymes of arginine biosynthesis
J. Mol. Biol.
(1971)
Y. Ebina et al.
Cyclic AMP-dependent initiation and θ-dependent termination of colicin E1 gene transcription
J. Biol. Chem.
(1983)
M.L. Gefter et al.
Role of modifications in tyrosine transfer RNA: A modified base affecting ribosome binding
J. Mol. Biol.
(1969)
C. Guerrier-Takada et al.
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
Cell
(1983)
T. Ikemura
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes
J. Mol. Biol.
(1981)
T. Ikemura
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for synonymous codon choice that is optimal for the E. coli translational system
J. Mol. Biol.
(1981)
T. Ikemura et al.
Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli
Cell
(1977)

A.I. Lamond et al.

Stringent control of bacterial transcription

Cell

(1985)

T. Maniatis et al.

Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7M urea

Meth. Enzymol.

(1980)

T. Mizuno et al.

A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)

H. Ohmori et al.

Structural analysis of the dnaA and dnaN genes of Escherichia coli

Gene

(1984)

R.D. Palmiter

Ovalbumin messenger ribonucleic acid translation

J. Biol. Chem.

(1973)

R.L. Pearson et al.

Improved separation of tRNA's on polychlorotrifluoroethylene-supported reversed-phase chromatography columns

Biochem. Biophys. Acta

(1971)

G. Peters et al.

Low-molecular-weight RNAs of Moloney Murine Leukemia Virus: Identification of the primer for RNA-directed DNA synthesis

J. Virol.

(1977)

J. Rossi et al.

The tyrT locus: termination and processing of a complex transcript

Cell

(1981)

G.B. Sancar et al.

The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters

Cell

(1982)

F. Sanger et al.

DNA sequencing with chain-terminating inhibitors

G. Zurawski et al.

Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon

L. Bossi et al.

The expression of prokaryotic tRNA genes in frog oocytes

Nucl. Acids Res.

(1983)

J. Carlson et al.

Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon

H. Donis-Keller

PhyM: an RNase activity specific for U and A residues useful in RNA sequence analysis

Nucl. Acids Res.

(1980)

H. Donis-Keller et al.

Mapping adenines, guanines, and pyrimidines in RNA

Nucl. Acids Res.

(1977)

Cited by (0)

^†: Present address: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544.

View full text

Cell

ArticleThe E. coli dnaY gene encodes an arginine transfer RNA

Abstract

J. Biol. Chem.

J. Biol. Chem.

Meth. Virol.

J. Mol. Biol.

J. Biol. Chem.

J. Mol. Biol.

Cell

J. Mol. Biol.

J. Mol. Biol.

Cell

Cell

Meth. Enzymol.

Gene

J. Biol. Chem.

Biochem. Biophys. Acta

J. Virol.

Cell

Cell

The expression of prokaryotic tRNA genes in frog oocytes

Nucl. Acids Res.

Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon

PhyM: an RNase activity specific for U and A residues useful in RNA sequence analysis

Nucl. Acids Res.

Mapping adenines, guanines, and pyrimidines in RNA

Nucl. Acids Res.

Article
The E. coli dnaY gene encodes an arginine transfer RNA