Conversion factors for estimation of cell production rates of soil bacteria from [3H]thymidine and [3H]leucine incorporation
References (40)
Thymidine incorporation into soil bacteria
Soil Biology & Biochemistry
(1990)- et al.
Microbial numbers and activity in dried and rewetted arable soil under integrated and conventional management
Soil Biology & Biochemistry
(1992) - et al.
Growth rate of rhizosphere bacteria measured directly by the tritiated thymidine incorporation technique
Soil Biology & Biochemistry
(1989) - et al.
Measuring bacterial production via rate of incorporation of [3H]thymidine into DNA
Journal of Microbiological Methods
(1984) - et al.
The Dutch programme on soil ecology of arable farming systems. 2. Geogenesis, agricultural history, field site characteristics and present farming systems at the Lovinkhoeve experimental farm
Agriculture, Ecosystems and Environment
(1989) - et al.
Effects of radioactive labelling of macromolecules, disturbance of bacteria and adsorption of thymidine to sediment on the determination of bacterial growth rates in sediment with tritiated thymidine
Journal of Microbiological Methods
(1990) - et al.
Measurement of bacterial growth rates on the rhizoplane using 3H-thymidine incorporation into DNA
Plant and Soil
(1990) - et al.
The relationship between cell size and viability of soil bacteria
Microbial Ecology
(1987) - et al.
Determination of bacterioplankton biomass, net production and growth efficiency in the Southern Ocean
Marine Ecology Progress Series
(1991) - et al.
Protozoan grazing and bacterial production in stratified Lake Vechten estimated with fluorescently labeled bacteria and by thymidine incorporation
Applied and Environmental Microbiology
(1989)
Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine
Applied and Environmental Microbiology
Conversion factors relating thymidine uptake to growth rate with rhizosphere bacteria
Uptake and incorporation of thymidine by bacterial isolates from an upwelling environment
Applied and Environmental Microbiology
Simulation of nitrogen mineralization in belowground food webs of two winter wheat fields
Journal of Applied Ecology.
Microautoradiography-based enumeration of bacteria with estimates of thymidine-specific growth and production rates
Marine Ecology Progress Series
Tritiated thymidine incorporation and the growth of heterotrophic bacteria in warm core rings
Limnology and Oceanography
Seasonal patterns of bacterial production and biomass in intertidal sediments of the western Dutch Wadden Sea
Marine Ecology Progress Series
DNA synthesis and tritiated thymidine incorporation by heterotrophic freshwater bacteria in continuous culture
Applied and Environmental Microbiology
Thymidine incorporation by the microbial community of standing dead
Spartina alterniflora. Applied and Environmental Microbiology
Nitrogen sources for the growth of marine microalgea: role of dissolved free amino acids
Marine Ecology Progress Series
Cited by (42)
Plant biomass, soil microbial community structure and nitrogen cycling under different organic amendment regimes; a <sup>15</sup>N tracer-based approach
2016, Applied Soil EcologyCitation Excerpt :A part of those biomarkers were grouped according to the classification as shown in Table 2, to study the relative importance of specific types of PLFA biomarkers in principal component analyses. Microbial activity was determined by 14C-leucine incorporation into microbial proteins during a short (1 h) incubation period (Michel and Bloem, 1993). For a more detailed description see de Vries et al. (2006).
Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe
2016, Applied Soil EcologyCitation Excerpt :Fungal biomass was determined after staining with differential fluorescent stain (DFS; Bloem and Vos, 2004). Microbial growth rate was measured from the uptake of radiolabelled 3H-thymidine and 14C-leucine, used in DNA and protein synthesis, respectively (Michel and Bloem, 1993; Bloem and Bolhuis, 2006). Bacterial genetic diversity was determined using PCR-DGGE (Muyzer et al., 1993; Dilly et al., 2004).
Production-ecological modelling explains the difference between potential soil N mineralisation and actual herbage N uptake
2014, Applied Soil EcologyCitation Excerpt :Bacterial biomass was calculated from the bacterial cell volume. Bacterial growth rate was determined by the incorporation of [3H]thymidine and [14C]leucine into bacterial macromolecules (Bloem and Bolhuis, 2006; Michel and Bloem, 1993). Sieved (4 mm) and homogenised samples of approximately 200 g field-moist soil, adjusted to 60% water holding capacity, were incubated in plastic bags.
Ecosystem services in grassland associated with biotic and abiotic soil parameters
2010, Soil Biology and BiochemistryCitation Excerpt :Bacterial biomass was calculated from the bacterial cell volume. Bacterial growth rate was determined as the incorporation of [3H]thymidine into bacterial DNA and proteins respectively (Bloem and Bolhuis, 2006; Michel and Bloem, 1993). For a more detailed description, see De Vries et al. (2006).
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Present address: DLO-Research Institute for Livestock Feeding and Nutrition, P.O. Box 160, 8200 AD Lelystad, The Netherlands.