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The estimation of dehydrogenase activity in soil

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    Triphenyltetrazolium chloride (TTC) was used as a substrate for determination of dehydrogenase activity. The absorbance of the triphenylformazan (TPF) at 492 nm was measured (Benefield et al., 1977). The soil CAT activity was determined using H2O2 as substrate.

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    Each PLFA and the sum of all PLFAs are expressed as ng g−1 soil. The activity of dehydrogenase (DHA) (EC 1.1.1.1) was assayed using 1 g of soil, and the iodonitrotetrazolium formazan (INTF) in the samples was measured by a modification of the method reported by Benefield et al. (1977) and expressed as µg INTF g−1 dry soil h−1. Fluorescein diacetate (FDA) and arylamidase (EC 3.4.11.4) activities were assayed by the method of Green et al. (2006) and Acosta-Martínez and Tabatabai (2000), respectively.

  • Bioremediation of cadmium polluted soil using a novel cadmium immobilizing plant growth promotion strain Bacillus sp. TZ5 loaded on biochar

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    The numbers of microbes were conducted by the spread plate count method according to previous report (Liu et al., 2018), and the numbers of colony forming units (CFU) of bacteria and fungi were counted. Dehydrogenase activity was determined by the amount of triphenylformazan (TPF) at the wavelength of 492 nm and defined as mg TPF/g soil/24 h (Benefield et al., 1977). Acid phosphatase activity was evaluated the content of p-nitrophenol (pNP) release at the wavelength of 400 nm and expressed as mg pNP/g soil/h (Aarle and Plassard, 2010).

  • Intercropping of kura clover (Trifolium ambiguum M. Bieb) with prairie cordgrass (Spartina pectinata link.) enhanced soil biochemical activities and microbial community structure

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    However, N fertilization rates and intercropping of KC in 2018 significantly affected TN, with PCG-KC recorded significantly higher soil TN than PCG-0N (data not shown). The DHA (EC 1.1.1.1) enzyme activity was assayed by using 1 g of soil, and the iodonitrotetrazolium formazan (INTF) in the samples was measured by a modification of the method reported by Benefield et al. (1977) and expressed as μg INTF g−1 dry soil h−1. Fluorescein diacetate (FDA), arylamidase (EC 3.4.11.4), and urease (EC 3.5.1.5) enzyme activities were assayed by the method of Green et al. (2006), Acosta-Martínez and Tabatabai (2000) and Kandeler and Gerber (1988), respectively.

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