A new immunoassay based on fluorescence excitation by internal reflection spectroscopy
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Historical development of sensing technology
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2022, Advanced Biosensors for Virus Detection: Smart Diagnostics to Combat SARS-CoV-2A split aptamer (SPA)-based sandwich-type biosensor for facile and rapid detection of streptomycin
2021, Journal of Hazardous MaterialsCitation Excerpt :Another SPA fragment, SPA2STR (in pink), was labeled with Cy5.5 and mixed with samples in a solution for the sandwich-type recognition of STR. Because the intensity of the evanescent field decreased exponentially with distance away from the interface (Kronick and Little, 1975), only Cy5.5 molecules bound to the sensing surface were excited. In the presence of STR, the Cy5.5-labeled SPA2STR assembly formed a sandwich-type ternary with the surface-immobilized SPA1STR and STR on the fiber surface, emitting detectable fluorescence signals recorded by a built-in computer.
Nanoporous layer fiber biosensing platform for real time culture- and separation-free detecting bacterial pathogens and measuring their susceptibility to antibiotics
2020, Sensors and Actuators, B: ChemicalCitation Excerpt :After 15 min incubation, the mixture was introduced into the microfluidic cell to detect the fluorescent signal. Optical fibers serve as ideal cylindrical dielectric waveguides to produce evanescent wave biosensing transducers, as well as fluorescence collectors [12–14]. Previous studies demonstrated that the tapered fiber probe with a matching V number, and determining the mode capacity of the fiber probe, could significantly enhance the excitation and collection efficiency of the fluorescence [21,22].
Integrated multichannel all-fiber optofluidic biosensing platform for sensitive and simultaneous detection of trace analytes
2018, Analytica Chimica ActaCitation Excerpt :Evanescent wave fiber-optical microarray biosensors have received considerable attention because of their small size, good flexibility, low propagation loss, and high sensitivity and specificity [13]. Since the first use of evanescent wave excitation for fluorescence immunoassay by Kronick and Little [14], several evanescent wave-based fiber optic biosensors have been proposed and applied for the determination of trace targets on the basis of total internal reflect fluorescence [3,15,16]. For instance, RAPTOR and Analyte 2000, both fiber-optical microarray biosensors, had successfully been applied for the detection of multiple bacteria and organic pollutants [17–19].
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Present address: Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, Ca. 94720, U.S.A.