Elsevier

Experimental Neurology

Volume 91, Issue 2, February 1986, Pages 399-403
Experimental Neurology

Research note
On the diffusion of horseradish peroxidase into muscles and the “spurious” labeling of motoneurons

https://doi.org/10.1016/0014-4886(86)90079-8Get rights and content

Abstract

There is some evidence to suggest that the fascial sheaths of muscles are important in preventing the labeling of motoneurons that occurs, apparently, as a result of the diffusion of horseradish peroxidase (HRP) into the muscles. To test this possibility, Gelfoam soaked in HRP was implanted over flexor and extensor muscles in the proximal forelimb of the rat. When the fascial sheaths were damaged, labeled neurons were found in the motoneuronal pools of the exposed muscles; if intact, virtually no labeling of motoneurons was observed. These results suggest that, if intramuscular injections are to be used as a method for identifying motoneuronal pools, care should be taken when exposing the muscle to be injected, to ensure that surrounding muscles and their fascial sheaths are not damaged.

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This work was supported by the Medical Research Council of Canada and the J. P. Bickell Foundation.

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