Elsevier

Brain Research

Volume 67, Issue 2, 22 February 1974, Pages 219-238
Brain Research

Afferents to the rat caudoputamen studied with horseradish peroxidase. An evaluation of a retrograde neuroanatomical research method

https://doi.org/10.1016/0006-8993(74)90274-1Get rights and content

Abstract

The potential for using the physiologically occurring retrograde axonal transport of protein as a neuroanatomical tool for identifying the origin of neuronal connections within the adult central nervous system has been evaluated for the case of the rat caudoputamen. Following injections of a marker protein, in this case horseradish peroxidase (HRP), centering on but not limited solely to the caudoputamen, peroxidase labeled cell bodies could be identified in several cell territories known or thought to contain afferents to this structure. These included the pars compacta of the substantia nigra, the intralaminar and parafascicular nuclei of the thalamus, and the dorsal nucleus of the midbrain raphe. In cases where only the caudoputamen and external segment of the globus pallidus were labeled directly at the injection site, no peroxidase- containing cells could be identified in the neocortex or in the thalamus outside the intralaminar and parafascicular nuclei. The evidence presented suggests first, that the degree of localization at the injection site is compatible with approaching some problems in neuroanatomy; second, that anterograde transport of the marker protein, if it occurs, does not appear to confound the interpretation of retrogradely labeled cell bodies; third, that many, though not all, afferent cell populations can be identified; and fourth, that labeling by axons in passage does not appear to be a problem. A detailed description of the method, abilities and limitations of the technique, and sources of misinterpretation are also provided.

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