Purification and characterization of a liposomal-forming tetraether lipid fraction

https://doi.org/10.1016/0006-291X(90)91756-IGet rights and content

Abstract

Polar lipid E, PLE, a native tetraether lipid mixture from Sulfolobus acidocaldarius is shown to spontaneously form multilamellar liposomes in aqueous media. PLE lipids were isolated as a single fraction from the crude lipid extract of S. acidocaldarius on a reverse-phase column followed by TLC and methanol precipitation. The methanol-precipitated mixture was able to form liposomes, whereas the non-precipitated material did not. It has thus been demonstrated, for the first time, that tetraether lipids from S. acidocaldarius can form liposomes, per se, in aqueous media.

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Present address: NEN Products, DuPont 549 Albany Street Boston, MA 02118.

1

We follow Langworthy's nomenclature in this paper. Another often-used name for PLE is P2; see, for example, Gulik, et al (9).

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