BBA report
The presence of DNA molecules with a displacement loop in standard mitochondrial DNA preparations

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Abstract

In electron micrographs of standard closed circular duplex mtDNA preparations from chick liver, spread by the protein monolayer technique from solutions containing 40 or 76% formamide, up to 30% of the molecules contained a singled-stranded displacement loop, as depicted in Fig. 1. The average lengths of the loops and of the doublestranded sections between the attachment points were about the same and equivalent to 3.5% of the contour length of the mtDNA circle.

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Permanent address: Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.

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Postal address: Zernikelaan, Groningen (The Netherlands).

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Postal address: Jan Swammerdam Institute, Eerste Constantijn Huygensstraat 20, Amsterdam, (The Netherlands).

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