Modulation of lipoprotein lipase activity in mouse peritoneal macrophages by recombinant human tumor necrosis factor

https://doi.org/10.1016/0005-2760(88)90281-0Get rights and content

Abstract

Thioglycollate-elicated mouse peritoneal macrophages spontaneously secrete lipoprotein lipase during culture. Exposure of the cultures to 50 ng/ml of recombinant human tumor necrosis factor (rTNF) for 48 h resulted in a 69% reduction in lipoprotein lipase activity in the culture medium with a concomitant decrease in cellular enzyme activity. The decrease in enzyme activity was not the result of rTNF-dependent reduction in the total protein synthesis, since the presence of rTNF did not affect [3H]leucine incorporation into cellular proteins. The effect of rTNF on lipoprotein lipase was reversible; upon TNF withdrawal, enzyme activity returned to basal levels after 60 h. The reduction of lipoprotein lipase in rTNF-treated cultures could be completely prevented by preincubation with a specific antiserum against recombinant human TNF. The late onset of decrease of lipoprotein lipase (LPL) activity suggests that rTNF might induce a mediator, which in turn suppresses LPL production. While rTNF was very effective in reducing lipoprotein lipase activity in mouse peritoneal macrophages, it did not affect lipoprotein lipase activity when added to the murine J774 cell line and to CT2 macrophage-like cells, a variant of the J774 cell line.

References (26)

  • G. Friedman et al.

    Biochim. Biophys. Acta

    (1988)
  • D.I. Goldberg et al.

    Biochem. Biophys. Res. Commun.

    (1987)
  • O. Sopher et al.

    Immunol. Lett.

    (1987)
  • R.N. Melmed et al.

    Biochim. Biophys. Acta

    (1983)
  • P. Nilsson-Ehle et al.

    J. Lipid Res.

    (1976)
  • P. Belfrage et al.

    J. Lipid Res.

    (1969)
  • H. Knobler et al.

    Biochim. Biophys. Acta

    (1984)
  • O.H. Lowry et al.

    J. Biol. Chem.

    (1951)
  • H. Semb et al.

    J. Biol. Chem.

    (1987)
  • H. Nakagawa et al.

    Biochem. Biophys. Res. Commun.

    (1987)
  • J.X. Lin et al.

    J. Biol. Chem.

    (1987)
  • M. Kawakami et al.
  • J.R. Mahoney et al.

    J. Immunol.

    (1985)
  • Cited by (27)

    • Physiological regulation of lipoprotein lipase

      2014, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
      Citation Excerpt :

      The inhibitory effect of LPS may be partially conveyed by numerous cytokines released in response to LPS, including TNFα, interferon γ and IL-1β [254]. Inhibition of secreted LPL activity by TNFα has been observed in mouse peritoneal macrophages but not in J774.1, BMDM and human monocyte-derived macrophages [252,253,255]. Interferon γ decreases macrophage LPL at least in part via a reduction in LPL synthesis and gene transcription, possibly via CK2 and PI3K signaling and regulation of Sp1/Sp3 binding [256,257].

    • Calorie restriction in mice does not affect LDL reverse cholesterol transport in vivo

      2003, Biochemical and Biophysical Research Communications
      Citation Excerpt :

      Cell culture. Peritoneal macrophages were obtained from C57BL/6 mice after intraperitoneal injection of thioglycollate [26]. The 106 cells/well were seeded in minimal essential medium (MEM) containing 10% fetal bovine serum.

    • In CCR2<sup>-/-</sup> mice monocyte recruitment and egress of LDL cholesterol in vivo is impaired

      2003, Biochemical and Biophysical Research Communications
      Citation Excerpt :

      Cell culture. Peritoneal cells were obtained from C57BL/6 or CCR2−/− mice after intraperitoneal injection of thioglycollate [19]. The 2×106 cells/well were seeded in minimal essential medium (MEM) containing 0.1% fatty acid-free bovine serum albumin and incubated with [3H]CE-Intralipid for 24 h. [3H]cholesteryl oleate ([3H]CE) was evaporated and 1 ml Intralipid in PBS (1 mg triglyceride/ml) was added and cosonicated for 6×30″, using a 4-mm tip and the Braun-Sonic 3000 sonicator (Braun, Melsungen, Germany), at 50% output [16].

    View all citing articles on Scopus
    View full text