Effect of phosphatidylcholine and free fatty acids on the activity of pancreatic lipase-colipase

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Abstract

Mixed micelles of bile salt and phospholipids inhibit the lipase-colipase-catalysed hydrolysis of triacylglycerols. Free fatty acids can reverse this inhibition and reactivate lipase-colipase. This reactivation is either due to the formation of a high-affinity complex between lipase and colipase induced by free fatty acids and/or to a change of the quality of the interface. Lauric acid, oleic acid and linoleic acid are the most potent reactivators, while short-chain free fatty acids have no effect and long-chain, saturated free fatty acids inhibit the lipase-colipase activity further. The physiological relevance of these results is evident as the glyceride emulsion reaching the duodenum already contains free fatty acids due to the activity of lingual lipase in the stomach.

References (21)

  • B. Borgström et al.

    J. Lipid Res.

    (1979)
  • G. Pieroni et al.

    J. Biol. Chem.

    (1979)
  • B. Borgström

    Gastroenterology

    (1980)
  • M. Rovery et al.

    Biochim. Biophys. Acta

    (1978)
  • C. Erlanson et al.

    Biochim. Biophys. Acta

    (1973)
  • A. Larsson et al.

    Biochim. Biophys. Acta

    (1981)
  • P. Belfrage et al.

    J. Lipid Res.

    (1969)
  • B. Borgström et al.

    FEBS Lett.

    (1979)
  • T. Wieloch et al.

    J. Biol. Chem.

    (1982)
  • C. Mollay et al.

    Biochim. Biophys. Acta

    (1973)
There are more references available in the full text version of this article.

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