Uptake of liposomes by cultured mouse bone marrow macrophages: influence of liposome composition and size

https://doi.org/10.1016/0005-2736(91)90268-DGet rights and content

Abstract

A wide range of liposome compositions have previously been examined in vivo for their ability to affect the uptake of liposomes into cells of the reticuloendothelial (RE, mononuclear phagocyte) system (Allen, T.M. and Chonn, A. (1987) FEBS Lett. 223, 42–46; Allen et al. (1989) Biochim. Biophys. Acta 981, 27–35). In this study we have examined the ability of cultured murine bone marrow macrophages to endocytose liposomes of various compositions and have looked for correlations between the in vivo and the in vitro observations. Compounds which substantially decreased RE uptake of liposomes in vivo, such as monosialoganglioside (GM1) and a novel synthetic lipid derivative of polyethyleneglycol (PEG-PE), also greatly decreased liposome uptake by bone marrow macrophages in a concentration-dependent manner. Lipids which increase bilayer rigidity, such as sphingomyelin (SM) and cholesterol (CHOL), decreased both in vivo and in vitro uptake of liposomes. Likewise, positive correlations were observed between the in vivo behavior of liposomes containing phosphatidylserine (PS) or various gangliosides and the ability of these liposomes to be taken up by bone marrow macrophages. Total liposome uptake by macrophages increased with incubation time at 37°C while very little liposome association with the macrophages was observed at 4°C. Liposome uptake increased with liposome concentration and for liposomes composed of egg phosphatidylcholine (PC) uptake plateaued at 40 nmol lipid per mg cell protein. There was an inverse correlation between liposome size of extruded large unilamellar vesicles and their uptake by macrophages.

Reference (40)

  • JonahM.M. et al.

    Biochim. Biophys. Acta

    (1975)
  • AllenT.M. et al.

    FEBS Lett.

    (1987)
  • AllenT.M. et al.

    Biochim. Biophys. Acta

    (1989)
  • SchwendenerR.A. et al.

    Biochim. Biophys. Acta

    (1984)
  • PrattenM.K. et al.

    Biochim. Biophys. Acta

    (1986)
  • HsuM.J. et al.

    Biochim. Biophys. Acta

    (1982)
  • JulianoR.L. et al.

    Biochim. Biophys. Acta

    (1985)
  • DijkstraJ. et al.

    Exp. Cell Res.

    (1984)
  • DijkstraJ. et al.

    Biochim. Biophys. Acta

    (1985)
  • DerksenJ.T.P. et al.

    Biochim. Biophys. Acta

    (1988)
  • IvanovV.O. et al.

    Biochim. Biophys. Acta

    (1985)
  • SommermanE.F. et al.

    Biochem. Biophys. Res. Commun.

    (1984)
  • MayerL.D. et al.

    Biochim. Biophys. Acta

    (1986)
  • PetersonG.L.

    Anal. Biochem.

    (1977)
  • BradfordM.

    Anal. Biochem.

    (1976)
  • BartlettG.R.

    J. Biol. Chem.

    (1959)
  • JulianoR.L. et al.

    Biochim. Biophys. Acta

    (1984)
  • SchroitA.J. et al.

    Chem. Phys. Lipids

    (1986)
  • SteinY. et al.

    FEBS Lett.

    (1980)
  • GregoriadisG. et al.

    FEBS Lett.

    (1980)
  • Cited by (327)

    • Etiology of lipid-laden macrophages in the lung

      2023, International Immunopharmacology
    • An Epigallocatechin-3-gallate Formulation Developed for Endodontic Use: A Physicochemical and Biological Evaluation

      2021, Journal of Endodontics
      Citation Excerpt :

      Although EGCG is soluble in water, the aqueous form is not ideal for intracanal dressing because it is quickly absorbed by periapical tissues35. Thus, the proposed EGCG formulation used a viscous PEG 400 vehicle, which is a flexible polymer that was selected because it is biocompatible and allows the substance to be released slowly over an extended period of time36–38, allowing the paste to be retained for a longer time within the root canal, in addition to maintaining the stability of the material’s properties compared with the aqueous vehicle. In addition to the presence of the active ingredient (EGCG) and the vehicle (PEG 400), an intracanal dressing must present a radiopacifying agent (zinc oxide) so that it can be evidenced radiographically in order to assess complete root canal filling.

    View all citing articles on Scopus
    *

    Present address: Department of Biochemistry, University of British Columbia, Vancouver, B.C., Canada, V6T 1W5.

    View full text