Regular paper
Interaction of Triton X-100 and octyl glucoside with liposomal membranes at sublytic and lytic concentrations. Spectroscopic studies

https://doi.org/10.1016/0005-2736(90)90111-ZGet rights and content

Abstract

The molecular mechanism of the solubilisation of phospholipid bilayers by nonionic detergents was studied by turbidity changes, carboxyfluorescein fluorescence dequenching, steady-state and time-resolved fluorescence anisotropy of DPH, lifetime measurements, ANS binding and 31P-NMR. Particular attention has been paid to the effective detergent-to-lipid ratio in the lipid phase. The disturbance of the bilayer arrangement varies considerably for various detergents depending on the hydrophilic and lipophilic parts of the molecule. Small amounts of detergents with low CMC (e.g. Triton X-100) can even induce an optimisation of packing of the lipid molecules.

References (39)

  • A. Helenius et al.

    Biochim. Biophys. Acta

    (1975)
  • D. Lichtenberg

    Biochim. Biophys. Acta

    (1985)
  • J. Lasch et al.

    Anal. Biochem.

    (1983)
  • E. Racker

    Methods Enzymol.

    (1979)
  • G.D. Eytan

    Biochim. Biophys. Acta

    (1982)
  • P. Schurtenberger et al.

    Biochim. Biophys. Acta

    (1984)
  • T. Bayerl et al.

    Biochim. Biophys. Acta

    (1986)
  • H. Van Dael et al.

    Chem. Phys. Lipids

    (1984)
  • F. Szoka et al.

    Biochim. Biophys. Acta

    (1980)
  • J.Ch.M. Stewart

    Anal. Biochem.

    (1980)
  • H. Gaub et al.

    Chem. Phys. Lipids

    (1985)
  • M. Ameloot et al.

    Biophys. J.

    (1984)
  • K. Kinosita et al.

    Biophys. J.

    (1977)
  • G. Lipari et al.

    Biophys. J.

    (1980)
  • G.K. Radda et al.

    Biochim. Biophys. Acta

    (1972)
  • T. Parasassi et al.

    J. Biol. Chem.

    (1984)
  • J. Ruiz et al.

    Biochim. Biophys. Acta

    (1988)
  • C.J. O'Connor et al.

    Biochim. Biophys. Acta

    (1985)
  • T.D. Madden et al.

    Biochim. Biophys. Acta

    (1982)
  • Cited by (78)

    • Special issue for Klaus Gawrisch

      2023, Biophysical Journal
    • Investigations on the role of edge activator upon structural transitions in Span vesicles

      2021, Colloids and Surfaces A: Physicochemical and Engineering Aspects
      Citation Excerpt :

      Later, niosomes evolved as non-ionic surfactant-based vesicles employing cholesterol as a VB. Liposome and niosomes have criticized as drug delivery carriers on the account of lack of flexibility in vesicle bilayer [3–6]. It is important to point out that flexible bilayer facilitates the transport of vesicles through biological barriers and enables the discharge of drug payload at deeper anatomical locations [7–9].

    • Reconstitution of SNARE proteins into solid-supported lipid bilayer stacks and X-ray structure analysis

      2018, Biochimica et Biophysica Acta - Biomembranes
      Citation Excerpt :

      Firstly pure PC-PE-PS-Chol vesicle bilayer prepared by sonication (dhh = 37.52 Å) is thicker than the one prepared by size-exclusion (dhh = 36.08 Å). As they have the same lipid composition, the only appropriate explanation is that there was fairly abundant detergent (mainly n-OG) left in the bilayers, which inserted into the lipid bilayers in a wedge-like fashion [53] and lead to bilayer thinning [54]. Secondly for sVS vesicles of PC-PE-PS-Chol/ΔN (dhh = 38.28 Å) and PC-PE-PS-Chol/Syb (dhh = 36.76 Å), dhh is increased with respect to PC-PE-PS-Chol (protein-free control).

    View all citing articles on Scopus
    View full text