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The mechanism of liposomal damage by taurocholate

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Abstract

The stability of small unilamellar vesicles formed by egg-yolk phosphatidylcholine (PC) has been examined in the presence of sodium taurocholate. The permeability of the vesicular membrane changes as the total taurocholate concentration increases, until a transformation from mixed bile salt/PC vesicles to mixed micelles occurs. Based on experiments in which the bile salt-induced release of either hydrophilic (carboxyfluorescein) or hydrophobic (Bromothymol blue) probes was studied, and on fluorescence polarization of the probe 1,6-diphenyl-1,3,5-hexatriene and turbidity measurements, a two-step process for the initial stage of liposomal damage by taurocholate is postulated.

References (36)

  • M.H. Richards et al.

    Biochim. Biophys. Acta

    (1978)
  • R.N. Rowland et al.

    Biochim. Biophys. Acta

    (1980)
  • C.J. O'Connor et al.

    Biochim. Biophys. Acta

    (1985)
  • F.J. Castellino et al.

    Arch. Biochem. Biophys

    (1979)
  • G.R. Bartlett

    J. Biol. Chem.

    (1959)
  • M. Shinitzky et al.

    J. Biol. Chem.

    (1974)
  • B.R. Lentz et al.

    Biophys. J.

    (1979)
  • M.C. Carey et al.

    Am. J. Med.

    (1970)
  • D. Lichtenberg

    Biochim. Biophys. Acta

    (1985)
  • E. Ralston et al.

    Biochim. Biophys. Acta

    (1981)
  • C.J. O'Connor et al.

    Adv. Colloid Interface Sci.

    (1985)
  • P. schurtenberger et al.

    Biochim. Biophys. Acta

    (1984)
  • D. Lichtenberg et al.

    Biochim. Biophys. Acta

    (1983)
  • R. Saez et al.

    FEBS Lett.

    (1985)
  • A. Helenius et al.

    Biochim. Biophys. Acta

    (1975)
  • R.N. Rowland et al.

    Biochim. Biophys. Acta

    (1980)
  • D. Lichtenburg et al.

    Biochemistry

    (1979)
  • R. Schubert et al.

    Biochemistry

    (1986)
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