Formation and characterization of mixed micelles of the nonionic surfactant Triton X-100 with egg, dipalmitoyl, and dimyristoyl phosphatidylcholines

doi:10.1016/0003-9861(74)90305-1

Archives of Biochemistry and Biophysics

Volume 165, Issue 2, December 1974, Pages 764-773

https://doi.org/10.1016/0003-9861(74)90305-1 Get rights and content

Abstract

Mixed micelles of the nonionic surfactant Triton X-100 and egg phosphatidylcholine were isolated by column chromatography on 6% agarose and by centrifugation at 35,000g. It was found that egg phosphatidylcholine bilayers are able to incorporate Triton X-100 at molar ratios of Triton to phospholipid below about 1:1, whereas above a molar ratio of about 2:1 Triton/phospholipid all of the phospholipid is converted into mixed micelles. Mixed micelles at a molar ratio of about 10:1 Triton/phospholipid were found to be in the same size range as pure micelles of Triton X-100. The formation of mixed micelles with dipalmitoyl phosphatidylcholine at room temperature, when the phospholipid is below its thermotropic phase transition, is shown to require relatively high concentrations of Triton X-100. The point at which dimyristoyl phosphatidylcholine bilayers are converted to mixed micelles was found to be less clear cut than with egg phosphatidylcholine, but above a molar ratio of about 2:1 Triton/phospholipid, all of this phospholipid is also in mixed micelles. The relevance of these results to the solubilization of membrane-bound proteins with Triton X-100 and the action of phospholipase A₂, which hydrolyzes phosphatidylcholine when it is in mixed micelles with Triton X-100, is discussed.

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^☆: A preliminary report of these results was presented at the ICN-UCLA Winter Conferences on Molecular Biology (Membranes), Squaw Valley, California, March 10–15, 1974.

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Formation and characterization of mixed micelles of the nonionic surfactant Triton X-100 with egg, dipalmitoyl, and dimyristoyl phosphatidylcholines☆

Abstract

Arch. Biochem. Biophys

Biochim. Biophys. Acta

J. Colloid Sci

Chem. Phys. Lipids

Chem. Phys. Lipids

J. Biol. Chem

Biochim. Biophys. Acta

J. Lipid Res

Biochim. Biophys. Acta

J. Colloid Interface Sci

J. Biol. Chem

Biochim. Biophys. Acta

J. Lipid Res

J. Supramol. Struct