Enzyme-catalyzed free radical reactions with nicotinamide-adenine nucleotides: I. Lactate dehydrogenase-catalyzed chain oxidation of bound NADH by superoxide radicals

doi:10.1016/0003-9861(73)90529-8

Archives of Biochemistry and Biophysics

Volume 159, Issue 2, December 1973, Pages 873-879

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Abstract

The radiation-induced oxidation of NADH by Superoxide radicals proceeds in the presence of lactate dehydrogenase by a chain mechanism. The reaction is initiated by O₂⁻ radicals, and propagated by O₂. The chain length is a function of [NADH]/[lactate dehydrogenase], the concentration of O₂, the dose rate, and pH. The chain reaction can be inhibited by addition of ascorbic acid.

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Enzyme-catalyzed free radical reactions with nicotinamide-adenine nucleotides: I. Lactate dehydrogenase-catalyzed chain oxidation of bound NADH by superoxide radicals

Abstract

J. Biol. Chem

J. Mol. Biol

Int. J. Radiat. Phys. Chem

J. Appl. Radiat. Isotopes

Biochim. Biophys. Acta

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

The Radiation Chemistry of Water and Aqueous Solutions

Principles of Radiation Chemistry

Amer. J. Roentgenol. Radium Ther. Nucl. Med

Phil. Mag