Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases

doi:10.1016/0003-2697(91)90318-N

Analytical Biochemistry

Volume 195, Issue 2, June 1991, Pages 207-213

https://doi.org/10.1016/0003-2697(91)90318-N Get rights and content

Abstract

A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6–12 ml, yielding 4.5–18 mg of homogeneous biologically active mRNA.

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Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases

Abstract

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

FEBS Lett

Eur. J. Biochem

Biol. Membr. (Rus)

Science

Nucleic Acids Res

Nucleic Acids Res