Elsevier

Analytical Biochemistry

Volume 166, Issue 2, 1 November 1987, Pages 368-379
Analytical Biochemistry

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

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Abstract

A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations than in glycine-SDS-PAGE systems. A superior resolution of proteins, especially in the range between 5 and 20 kDa, is achieved without the necessity to use urea. Proteins above 30 kDa are already destacked within the sample gel. Thus a smooth passage of these proteins from sample to separating gel is warranted and overloading effects are reduced. This is of special importance when large amounts of protein are to be loaded onto preparative gels. The omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.

References (19)

  • H. Schägger et al.
  • D.M. Neville et al.
  • M. Douglas et al.
  • R.T. Swank et al.

    Anal. Biochem

    (1971)
  • M. Wyckoff et al.

    Anal. Biochem

    (1977)
  • F.W. Studier

    J. Mol. Biol

    (1973)
  • J. Kyte et al.

    Anal. Biochem

    (1983)
  • F. Cabral et al.
  • D. Bothe et al.

    Anal. Biochem

    (1985)
There are more references available in the full text version of this article.

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