Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels

doi:10.1016/0003-2697(83)90551-1

Analytical Biochemistry

Volume 129, Issue 2, March 1983, Pages 277-287

https://doi.org/10.1016/0003-2697(83)90551-1 Get rights and content

A sodium dodecyl sulfate-discontinuous Polyacrylamide gel electrophoresis system for separation and quantitation of low-molecular-weight (75 to 10K Da) proteins from single muscle fibers is described. Slab gels (0.75 mm thick) were stained using an improved silver-stain technique which does not require photographic fixers in order to achieve low-level background staining. The modified staining procedure uses continuous flow washing to minimize the handling of gels. The procedure has high sensitivity and gave a linear response between approximately 2 and 70 ng of protein per band. In addition, a convenient method for mounting slab gels for photography, scanning, and long-term storage has been developed.

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Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels

J. Biol. Chem.

Anal. Biochem.

Anal. Biochem.

Anal. Biochem.

Arch. Biochem. Biophys.

Electrophoresis

Electrophoresis